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Biochemical Large-Scale Identification of MHC Class I Ligands

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Antigen Processing

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 960))

Abstract

The large-scale identification of MHC class I presented peptides is indispensable for gaining insight into the fundamental rules of immune recognition as well as it is an invaluable tool in identifying potential targets for the immunotherapy of disease. In this chapter we briefly review the existing strategies for the analysis of MHC ligandomes and provide an in-depth protocol for the immunoaffinity purification of MHC class I presented peptides from primary tissues or cells.

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Author information

Authors and Affiliations

  1. Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany

    Daniel J. Kowalewski

  2. Department of Immunology, University of Tübingen, Interfaculty Institute for Cell Biology, Tübingen, Germany

    Stefan Stevanović

Authors
  1. Daniel J. Kowalewski
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  2. Stefan Stevanović
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Corresponding author

Correspondence to Stefan Stevanović .

Editor information

Editors and Affiliations

  1. Institut National de la Santé et de la Recherche Médicale, Unité 1013, Université Paris Descartes, Faculté de médecine, Paris, France

    Peter van Endert

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Kowalewski, D.J., Stevanović, S. (2013). Biochemical Large-Scale Identification of MHC Class I Ligands. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology™, vol 960. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-218-6_12

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  • DOI: https://doi.org/10.1007/978-1-62703-218-6_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-217-9

  • Online ISBN: 978-1-62703-218-6

  • eBook Packages: Springer Protocols

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