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Merging of Image Data in Electron Crystallography

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Electron Crystallography of Soluble and Membrane Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 955))

Abstract

Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to record images and diffraction patterns of frozen-hydrated 2D crystals. Each two-dimensional (2D) crystal is only imaged once, at one specific tilt angle, and the recorded images can be automatically processed with the 2dx/MRC software package. Processed image data from non-tilted and tilted 2D crystals then need to be merged into a 3D reconstruction of the membrane protein structure. We here describe the process of the 3D merging, using the 2dx software system.

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Acknowledgments

This work was in part supported by the Swiss initiative for Systems Biology (SystemsX.ch, grant CINA), Swiss National Science Foundation (Grant 205321_126490), the NCCRs TransCure, Nano, and Structural Biology.

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Authors and Affiliations

  1. C-CINA, Biozentrum, University Basel, Basel, Switzerland

    Marcel Arheit, Daniel Castaño-Diéz, Raphaël Thierry, Priyanka Abeyrathne & Henning Stahlberg

  2. Department of Computer Sciences, Rice University, Houston, TX, USA

    Bryant R. Gipson

Authors
  1. Marcel Arheit
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  2. Daniel Castaño-Diéz
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  3. Raphaël Thierry
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  4. Priyanka Abeyrathne
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  5. Bryant R. Gipson
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  6. Henning Stahlberg
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Corresponding author

Correspondence to Henning Stahlberg .

Editor information

Editors and Affiliations

  1. School of Biology, Georgia Institute of Technology, Ferst Dr. 310, Atlanta, 30332, Georgia, USA

    Ingeborg Schmidt-Krey

  2. , Dept. Biochemistry & Biophysics, University of California, San Francisco, 16th St. 600, San Francisco, 94158, California, USA

    Yifan Cheng

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Arheit, M., Castaño-Diéz, D., Thierry, R., Abeyrathne, P., Gipson, B.R., Stahlberg, H. (2013). Merging of Image Data in Electron Crystallography. In: Schmidt-Krey, I., Cheng, Y. (eds) Electron Crystallography of Soluble and Membrane Proteins. Methods in Molecular Biology, vol 955. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-176-9_11

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  • DOI: https://doi.org/10.1007/978-1-62703-176-9_11

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-175-2

  • Online ISBN: 978-1-62703-176-9

  • eBook Packages: Springer Protocols

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