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Identification of Legionella Effectors Using Bioinformatic Approaches

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Legionella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 954))

Abstract

Legionella pneumophila the causative agent of Legionnaires’ disease, actively manipulates host cell processes to establish a replication niche inside host cells. The establishment of its replication niche requires a functional Icm/Dot type IV secretion system which translocates about 300 effector proteins into host cells during infection. Many of these effectors were first identified as effector candidates by several bioinformatic approaches, and these predicted effectors were later examined experimentally for translocation and a large number of which were validated as effector proteins. Here, I summarized the bioinformatic approaches that were used to identify these effectors.

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Author information

Authors and Affiliations

  1. George S. Wise Faculty of Life Sciences, Department of Molecular MIcrobiology and Biotechnology, Tel-Aviv University, Ramat-Aviv, Israel

    Gil Segal

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  1. Gil Segal
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Correspondence to Gil Segal .

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Editors and Affiliations

  1. , Biology of Intracellular Bacteria, Institut Pasteur, Rue du Dr. Roux 28, Paris, 75724, France

    Carmen Buchrieser

  2. Max von Pettenkofer Institute, Ludwig-Maximilians-University, Pettenkoferstrasse 9a, Munich, 80336, Germany

    Hubert Hilbi

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Segal, G. (2013). Identification of Legionella Effectors Using Bioinformatic Approaches. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 954. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-161-5_37

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  • DOI: https://doi.org/10.1007/978-1-62703-161-5_37

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-160-8

  • Online ISBN: 978-1-62703-161-5

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