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Quantitative Analysis of Glycoprotein Glycans

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Mass Spectrometry of Glycoproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 951))

Abstract

The ability to quantitatively determine changes in the N- and O-linked glycans is an essential component of comparative glycomics. Multiple strategies are available to by which this can be accomplished, including; both label free approaches and isotopic labeling strategies. The focus of this chapter is to describe each of these approaches while providing insight into their strengths and weaknesses, so that glycomic investigators can make an educated choice of the strategy that is best suited for their particular application.

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Author information

Authors and Affiliations

  1. Departments of Biochemistry and Molecular Biology, and Chemistry, University of Georgia, Athens, GA, USA

    Ron Orlando

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  1. Ron Orlando
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Correspondence to Ron Orlando .

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Editors and Affiliations

  1. Medical Center, Department of Biochemistry, University of Texas Southwestern, Harry Hines Blvd. 5323, Dallas, 75390-9038, Texas, USA

    Jennifer J. Kohler

  2. Medical Center, Dallas, University of Texas Southwestern, Harry Hines Blvd. 5323, Dallas, 75390-9185, Texas, USA

    Steven M. Patrie

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Orlando, R. (2013). Quantitative Analysis of Glycoprotein Glycans. In: Kohler, J., Patrie, S. (eds) Mass Spectrometry of Glycoproteins. Methods in Molecular Biology, vol 951. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-146-2_13

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  • DOI: https://doi.org/10.1007/978-1-62703-146-2_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-145-5

  • Online ISBN: 978-1-62703-146-2

  • eBook Packages: Springer Protocols

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