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Laboratory Maintenance of Rodent Malaria Parasites

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Malaria

Part of the book series: Methods in Molecular Biology ((MIMB,volume 923))

Abstract

We provide a series of protocols that have been used for the cyclic transmission of rodent malaria parasites in the laboratory. This is now possible both in vivo and in vitro. We focus on the least “resource intensive” and generic methods that we find applicable to any parasite–host combination. Nonetheless, we recognize that the ability to construct transgenic “reporter” parasites/hosts now permits the use of elegant analytical and imaging technologies both in vitro, ex vivo, and in vivo in specific instances. The descriptions given illustrate methods routinely used for the maintenance of P. berghei; where critical, we note important differences when transmitting other parasite species.

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References

  1. Suhrbier A et al (1987) The complete development in vitro of the vertebrate phase of the mammalian malarial parasite Plasmodium berghei. Trans R Soc Trop Med Hyg 81:907–909

    Article  PubMed  CAS  Google Scholar 

  2. Al-Olayan EM et al (2002) Complete development of mosquito phases of the malaria parasite in vitro. Science 295:677–679

    Article  PubMed  CAS  Google Scholar 

  3. Vanderberg JP, Gwadz RW (1980) The transmission by mosquitoes of plasmodia in the laboratory. In: Kreier JP (ed) Malaria, vol 2, Pathology, vector studies, and culture. Academic, New York, pp 154–234

    Google Scholar 

  4. Sinden RE (1996) Infection of mosquitoes with rodent malaria. In: Crampton JM, Beard CB, Louis C (eds) Molecular biology of insect disease vectors: a methods manual, 1st edn. Chapman & Hall, London, pp 67–91

    Google Scholar 

  5. Sinden RE et al (2002) Maintenance of the Plasmodium berghei life cycle. In: Doolan D (ed) Methods in molecular medicine. Humana, Totowa, NJ, pp 25–40

    Google Scholar 

  6. Shute P, Maryon M (1966) Laboratory technique for the study of malaria, 2nd edn. J & A Churchill, London

    Google Scholar 

  7. Franke-Fayard B et al (2004) A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle. Mol Biochem Parasitol 137:23–33

    Article  PubMed  CAS  Google Scholar 

  8. Dearsly AL et al (1990) Sexual development in malarial parasites: gametocyte production, fertility and infectivity to the mosquito vector. Parasitology 100:359–368

    Article  PubMed  Google Scholar 

  9. Jaffe RI et al (1990) Differences in susceptibility among mouse strains to infection with Plasmodium berghei (ANKA clone) sporozoites and its relationship to protection by gamma-irradiated sporozoites. Am J Trop Med Hyg 42:309–313

    PubMed  CAS  Google Scholar 

  10. Scheller LF et al (1994) Susceptibility of different strains of mice to hepatic infection with Plasmodium berghei. Infect Immun 62:4844–4847

    PubMed  CAS  Google Scholar 

  11. Mons B (1986) Intraerythrocytic differentiation of Plasmodium berghei. Acta Leiden 54:1–83

    PubMed  CAS  Google Scholar 

  12. Schneider I (1972) Cell lines derived from late embryonic stages of Drosophila melanogaster. J Embryol Exp Morphol 27:353–365

    PubMed  CAS  Google Scholar 

  13. Vanderberg JP et al (1968) Protective immunity produced by the injection of x-irradiated sporozoites of Plasmodium berghei. II. Effects of radiation on sporozoites. J Parasitol 54:175–180

    Article  Google Scholar 

  14. Sinden RE et al (2008) Progression of Plasmodium berghei through Anopheles stephensi is density-dependent. PLoS Pathog 3:e195

    Article  Google Scholar 

  15. Ploemen IH et al (2009) Visualisation and quantitative analysis of the rodent malaria liver stage by real time imaging. PLoS One 4:e7881

    Article  PubMed  Google Scholar 

  16. Feldmann AM, Ponnudurai T (1989) Selection of Anopheles stephensi for refractoriness and susceptibility to Plasmodium falciparum. Med Vet Entomol 3:41–52

    Article  PubMed  CAS  Google Scholar 

  17. Sato Y et al (1996) Effect of temperature on Plasmodium berghei and P. yoelii on mosquito stage in Anopheles stephensi. J Parasitol 45:98–104

    Google Scholar 

  18. Yoeli M, Upmanis RS (1968) Plasmodium berghei ookinete formation in vitro. Exp Parasitol 22:122–128

    Article  PubMed  CAS  Google Scholar 

  19. Strome CP et al (1979) The cultivation of the exoerythrocytic stages of Plasmodium berghei from sporozoites. In Vitro 15:531–536

    Article  PubMed  CAS  Google Scholar 

  20. Sinden RE, Smith JE (1980) Culture of the liver stages (exoerythrocytic schizonts) of rodent malaria parasites from sporozoites in vitro. Trans R Soc Trop Med Hyg 74:134–136

    Article  PubMed  CAS  Google Scholar 

  21. Calvo-Calle JM et al (1994) In vitro development of infectious liver stages of P. yoelii and P. berghei malaria in human cell lines. Exp Parasitol 79:362–373

    Article  PubMed  CAS  Google Scholar 

  22. Sinden RE et al (1990) The development and routine application of high-density exoerythrocytic-stage cultures of Plasmodium berghei. Bull World Health Organ 68:115–125

    PubMed  Google Scholar 

  23. Davies CS et al (1989) Improved techniques for the culture of the liver stages of Plasmodium berghei and their relevance to the study of causal prophylactic drugs. Acta Leiden 58:97–113

    PubMed  CAS  Google Scholar 

  24. Mazier D et al (1982) Infection in vitro d’hépatocytes de Thamnomys adultes par des sporozoïtes de P. yoelii: développement de schizontes et libération de mérozoïtes infectants. Ann Parasitol Hum Comp 57:99–100

    PubMed  CAS  Google Scholar 

  25. Hollingdale MR et al (1985) In vitro cultivation of the exoerythrocytic stage of Plasmodium berghei in irradiated hepatoma cells. Am J Trop Med Hyg 34:21–23

    PubMed  CAS  Google Scholar 

  26. Mons B et al (1983) In vitro culture of Plasmodium berghei using a new suspension system. Int J Parasitol 13:213–217

    Article  PubMed  CAS  Google Scholar 

  27. Waters AP et al (1997) Transfection of malaria parasites. Methods 13:134–147

    Article  PubMed  CAS  Google Scholar 

  28. Billker O et al (2004) Calcium and a calcium-dependent protein kinase regulate gamete formation and mosquito transmission in a malaria parasite. Cell 117:503–514

    Article  PubMed  CAS  Google Scholar 

  29. Mishell B, Shiigi S (1980) Selected methods in cellular immunology. W.H. Freeman & Co, San Fransico, USA, pp 23–24

    Google Scholar 

  30. Delves M, Sinden RE (2010) A semi-automated method for counting fluorescent malaria oocysts increases the throughput of transmission blocking studies. Malar J 9:35

    Article  PubMed  Google Scholar 

  31. Motard A et al (1993) The role of reactive nitrogen intermediates in modulation of gametocyte infectivity of rodent malaria parasites. Parasite Immunol 15:21–26

    Article  PubMed  CAS  Google Scholar 

  32. Gautret P et al (1996) The gametocytes of Plasmodium vinckei petteri, their morphological stages, periodicity and infectivity. Int J Parasitol 26:1095–1101

    PubMed  CAS  Google Scholar 

  33. Gautret P et al (2000) The effects of subcurative doses of chloroquine on Plasmodium vinckei petteri gametocytes and on their infectivity to mosquitoes. Int J Parasitol 30:1193–1198

    Article  PubMed  CAS  Google Scholar 

  34. Buckling AG et al (1997) Adaptive changes in Plasmodium transmission strategies following chloroquine chemotherapy. Proc Biol Sci 264:553–559

    Article  PubMed  CAS  Google Scholar 

  35. Buckling AG, Read AF (1999) The effect of chloroquine treatment on the infectivity of Plasmodium chabaudi gametocytes. Int J Parasitol 29:619–625

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. The Malaria Centre, Department of Life Sciences, Imperial College, London, UK

    Chandra Ramakrishnan, Michael J. Delves, Kalpana Lal, Andrew M. Blagborough, Geoffrey Butcher, Kenneth W. Baker & Robert E. Sinden

Authors
  1. Chandra Ramakrishnan
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  2. Michael J. Delves
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  3. Kalpana Lal
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  4. Andrew M. Blagborough
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  5. Geoffrey Butcher
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  6. Kenneth W. Baker
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  7. Robert E. Sinden
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Corresponding author

Correspondence to Robert E. Sinden .

Editor information

Editors and Affiliations

  1. , Malaria Biology and Genetics Unit, Institut Pasteur, Rue du Dr Roux 28, Paris, 75724, France

    Robert Ménard

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Ramakrishnan, C. et al. (2012). Laboratory Maintenance of Rodent Malaria Parasites. In: Ménard, R. (eds) Malaria. Methods in Molecular Biology, vol 923. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-026-7_5

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  • DOI: https://doi.org/10.1007/978-1-62703-026-7_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-025-0

  • Online ISBN: 978-1-62703-026-7

  • eBook Packages: Springer Protocols

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