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Mosquito Transgenic Technologies to Reduce Plasmodium Transmission

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Malaria

Part of the book series: Methods in Molecular Biology ((MIMB,volume 923))

Abstract

The ability to introduce genetic constructs of choice into the genome of Anopheles mosquitoes provides a valuable tool to study the molecular interactions between the Plasmodium parasite and its insect host. In the long term, this technology could potentially offer new ways to control vector-borne diseases through the suppression of target mosquito populations or through the introgression of traits that preclude pathogen transmission. Here, we describe in detail protocols for the generation of transgenic Anopheles gambiae mosquitoes based on germ-line transformation using either modified transposable elements or the site-specific PhiC31 recombinase.

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Author information

Authors and Affiliations

  1. Department of Life Sciences, Imperial College, London, UK

    Silke Fuchs, Tony Nolan & Andrea Crisanti

Authors
  1. Silke Fuchs
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  2. Tony Nolan
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  3. Andrea Crisanti
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Corresponding author

Correspondence to Andrea Crisanti .

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Editors and Affiliations

  1. , Malaria Biology and Genetics Unit, Institut Pasteur, Rue du Dr Roux 28, Paris, 75724, France

    Robert Ménard

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Fuchs, S., Nolan, T., Crisanti, A. (2012). Mosquito Transgenic Technologies to Reduce Plasmodium Transmission. In: Ménard, R. (eds) Malaria. Methods in Molecular Biology, vol 923. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-026-7_41

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  • DOI: https://doi.org/10.1007/978-1-62703-026-7_41

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-025-0

  • Online ISBN: 978-1-62703-026-7

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