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Using the Multifunctional Xylem Probe for in situ Studies of Plant Water and Ion Relations Under Saline Conditions

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Plant Salt Tolerance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 913))

Abstract

By insertion into an individual xylem vessel at the root base, the multifunctional xylem probe allows the monitoring of the xylem pressure, the radial electrical gradients in the root (the so-called trans-root potential, TRP), as well as the activity of a particular ion such as K+ in the xylem sap of intact, transpiring plants. The biophysical and physiological significance of these parameters with respect to salt stress is briefly explained, and the assembly of the probe, the setup used for these measurements, and the experimental procedure are outlined in detail.

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Author information

Authors and Affiliations

  1. Karlsruhe Institute of Technology, Institute of Botany I and Institute of Pulsed Power and Microwave Technology, Eggenstein-Leopoldshafen, Germany

    Lars H. Wegner

Authors
  1. Lars H. Wegner
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Correspondence to Lars H. Wegner .

Editor information

Editors and Affiliations

  1. School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, 7001, Tasmania, Australia

    Sergey Shabala

  2. INRA-SupAgro, BPMP-IBIPGC, place Viala 1, Montpellier, 34060, France

    Tracey Ann Cuin

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Wegner, L.H. (2012). Using the Multifunctional Xylem Probe for in situ Studies of Plant Water and Ion Relations Under Saline Conditions. In: Shabala, S., Cuin, T. (eds) Plant Salt Tolerance. Methods in Molecular Biology, vol 913. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-986-0_3

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  • DOI: https://doi.org/10.1007/978-1-61779-986-0_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-985-3

  • Online ISBN: 978-1-61779-986-0

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