Abstract
Teeth contain an incremental record of their growth in the form of periodic growth markings that are literally embodied within the structure of enamel. These can be revealed when ground sections of teeth are prepared for transmitted light microscopy. This chapter sets out one method for estimating the time taken to form the crowns of permanent teeth. First, the daily rates of enamel formation are estimated at three positions within the crown close to the enamel dentine junction (EDJ). Then a 200 μm length along an enamel prism that take ∼80 days to form is followed out from the EDJ at the dentine horn. From this point a line is then tracked back along the direction of the oblique striae of Retzius that are contained within enamel to a lower position further along the EDJ. This procedure is repeated until the cervix of the tooth is reached. The total time for enamel formation is estimated by summing the number of 80 day segments traced out.
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References
Liversidge HM (2009) Permanent tooth formation as a method of estimating age. In: Koppe T, Meyer G, Alt KW (eds) Frontiers of Oral Biology; Interdisciplinary Dental Morphology. Karger, Basel
Bromage TG (1991) Enamel incremental periodicity in the pig-tailed macaque; a polychrome fluorescent labelling study of dental hard tissues. Am J Phys Anthropol 86:205–214
Bromage TG, Lacruz RS, Hogg R, Goldman HM, McFarlin SC, Warshaw J, Dirks W, Perez-Ochon A, Smolyar I, Enlow D, Boyde A (2009) Lamellar Bone is an incremental tissue reconciling enamel rhythms, body size and organismal life history. Calcif Tissue Int 84(5):388–404
Smith TM (2006) Experimental determination of the periodicity of incremental features in enamel. J Anat 208:99–113
Boyde A (1976) Amelogenesis and the strucvture of enamel. In: Kramer IRH, Cohen B (eds) Scientific Foundations of Dentistry. W. Heinemann Medical Books Ltd., London
Boyde A (1979) Carbonate concentration, crystal centres, core dissolution, caries, cross striation, circadian rhythms and compositional contrast in the SEM. J Dent Res 58:981–983
Boyde A (1989) Enamel. In: Oksche A, Vollrath L (eds) Handbook of microscopic anatomy, vol 2, Teeth, Springer, Berlin
Boyde A (1990a) Developmental interpretations of dental microstructure. In: De Rousseau, Jean C (eds) Primate life history and evolution: monographs in primatology, vol 14. Wiley-Liss Inc, New York
Shinoda H (1984) Faithful records of biological rhythms in dental hard tissues. Chem Today 162:34–40 (In Japanese)
Boyde A (1990) Confocal optical microscopy. In: Duke PJ, Michette AG (eds) Modern microscopies: techniques and applications. Plenum Press, New York
Dean MC (2009) Growth in tooth height and extension rates in modern human and fossil hominin canines and molars. In: Koppe T, Meyer G, Alt KW (eds) Frontiers of Oral Biology; Interdisciplinary Dental Morphology. Karger, Basel
Antoine D, Hillson S, Dean MC (2009) The developmental clock of dental enamel: A test for the periodicity of prism cross-striations and an evaluation of the likely sources of error in histological studies of this kind. J Anat 214:45–55
Retzius A (1837) Bemerkungen über den inneren Bau der Zähne, mit besonderer Rücksicht auf dem in Zahnknochen vorkommenden Röhrenbau. (Müllers) Arch Anat Physiol 1837:486–566
Boyde A (1963) Estimation of age at death of young human skeletal remain from incremental lines in dental enamel. Third International Meeting in Forensic Immunology, Medicine, Pathology and Toxicology, pp 36–46
Boyde A (1964) The structure and development of mammalian enamel. PhD Thesis, University of London
Shellis RP (1984) Variations in growth of the enamel crown in human teeth and a possible relationship between growth and enamel structure. Arch Oral Biol 29:697–705
Risnes S (1986) Enamel apposition rate and the prism periodicity in human teeth. Scand J Dent Res 94:394–404
Reid DJ, Dean MC (2005) Variation in modern human enamel formation times. J Hum Evol 50:329–346
Mahoney P (2008) Intraspecific variation in M1 enamel development in modern humans: Implications for human evolution. J Hum Evol 55:130–146
Dean C, Leakey MG, Reid D, Schrenk F, Schwartz GT, Stringer C, Walker A (2001) Growth processes in teeth distinguish modern humans from Homo erectus and earlier hominins. Nature 414:628–631
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Dean, M.C. (2012). A Histological Method That Can Be Used to Estimate the Time Taken to Form the Crown of a Permanent Tooth. In: Bell, L. (eds) Forensic Microscopy for Skeletal Tissues. Methods in Molecular Biology, vol 915. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-977-8_5
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DOI: https://doi.org/10.1007/978-1-61779-977-8_5
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