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Production of Antibody Fragments in Escherichia coli

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Antibody Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 907))

Abstract

Escherichia coli is a host widely used in the industrial production of recombinant proteins. However, the expression of heterologous proteins in E. coli often encounters the formation of inclusion bodies, which are insoluble and nonfunctional protein aggregates. For the successful production of antibody fragments, which includes single-chain variable fragments (scFvs), we describe here the modification of linker, signal, and Shine–Dalgarno (SD) sequences, the coexpression of cytoplasmic and periplasmic chaperones, and a method for fed-batch cultivation with exponential feed.

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Author information

Authors and Affiliations

  1. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan

    Tomohisa Katsuda & Hideki Yamaji

  2. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University and Research Institute, Kobe, Japan

    Hiroyuki Sonoda

  3. Research and Development Division, JCR Pharmaceuticals Co., Ltd, Kobe, Japan

    Hiroyuki Sonoda

  4. Department of Chemistry and Materials Technology, Kyoto Institute of Technology, Kyoto, Japan

    Yoichi Kumada

Authors
  1. Tomohisa Katsuda
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  2. Hiroyuki Sonoda
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  3. Yoichi Kumada
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  4. Hideki Yamaji
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Corresponding author

Correspondence to Tomohisa Katsuda .

Editor information

Editors and Affiliations

  1. Antibody Therapeutics & Immunotargeting, INSERM U624, Marseille CEDEX 09, France

    Patrick Chames

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© 2012 Springer Science+Business Media, LLC

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Katsuda, T., Sonoda, H., Kumada, Y., Yamaji, H. (2012). Production of Antibody Fragments in Escherichia coli . In: Chames, P. (eds) Antibody Engineering. Methods in Molecular Biology, vol 907. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-974-7_18

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  • DOI: https://doi.org/10.1007/978-1-61779-974-7_18

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-973-0

  • Online ISBN: 978-1-61779-974-7

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