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Use of IMGT® Databases and Tools for Antibody Engineering and Humanization

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Antibody Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 907))

Abstract

IMGT®, the international ImMunoGeneTics information system® (http://www.imgt.org), was created in 1989 to manage the huge diversity of the antigen receptors, immunoglobulins (IG) or antibodies, and T cell receptors (TR). Standardized sequence and structure analysis of antibody using IMGT® databases and tools allows one to bridge, for the first time, the gap between antibody sequences and three-dimensional (3D) structures. This is achieved through the IMGT Scientific chart rules, based on the IMGT-ONTOLOGY concepts of classification (IMGT gene and allele nomenclature), description (IMGT standardized labels), and numerotation (IMGT unique numbering and IMGT Colliers de Perles). IMGT® is the international reference for immunogenetics and immunoinformatics and its standards are particularly useful for antibody humanization and evaluation of immunogenicity. IMGT® databases for antibody nucleotide sequences and genes include IMGT/LIGM-DB and IMGT/GENE-DB, respectively, whereas nucleotide sequence analysis is performed by the IMGT/V-QUEST, IMGT/HighV-QUEST, and IMGT/JunctionAnalysis tools. In this chapter, we focus on IMGT® databases and tools for amino acid sequences, two-dimensional (2D) and three-dimensional (3D) structures: the IMGT/DomainGapAlign and IMGT/Collier-de-Perles tools, the IMGT/2Dstructure-DB database for amino acid sequences of monoclonal antibodies (mAb, suffix -mab) and fusion proteins for immune applications (FPIA, suffix -cept) of the World Health Organization/International Nonproprietary Name (WHO/INN) programme and other proteins of interest, and the IMGT/3Dstructure-DB database for crystallized antibodies and its associated tools (IMGT/StructuralQuery, IMGT/DomainSuperimpose).

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References

  1. Lefranc M-P, Giudicelli V, Ginestoux C, Jabado-Michaloud J, Folch G, Bellahcene F, Wu Y, Gemrot E, Brochet X, Lane J, Regnier L, Ehrenmann F, Lefranc G, Duroux P (2009) IMGT®, the international ImMunoGeneTics information system®. Nucl Acids Res 37:D1006–D1012

    Article  CAS  PubMed  Google Scholar 

  2. Lefranc M-P, Lefranc G (2001) The immunoglobulin FactsBook. Academic Press, London, pp 1–458

    Google Scholar 

  3. Lefranc M-P, Lefranc G (2001) The T cell receptor FactsBook. Academic Press, London, UK, pp 1–398

    Google Scholar 

  4. Lefranc M-P (2011) IMGT, the international ImMunoGeneTics information system. Cold Spring Harb Protoc 2011(6):595–603. doi:10.1101/pdb.top115

    PubMed  Google Scholar 

  5. Lefranc M-P (2013) IMGT® information system. In: Dubitzky W, Wolkenhauer O, Yokota H, Cho KH (ed) Encyclopedia of Systems Biology, Springer Science  +  Business Media, Dordrecht, Netherlands doi: 10.1007/978-1-4419-9863-7, LLC012 (in press)

  6. Lefranc M-P (2007) WHO-IUIS nomenclature subcommittee for immunoglobulins and T cell receptors report. Immunogenetics 59:899–902

    Article  PubMed  Google Scholar 

  7. Lefranc M-P (2008) WHO-IUIS nomenclature subcommittee for immunoglobulins and T cell receptors report august 2007, 13th international congress of immunology, Rio de Janeiro. Brazil Dev Comp Immunol 32:461–463

    Article  Google Scholar 

  8. Giudicelli V, Lefranc M-P (1999) Ontology for immunogenetics: IMGT-ONTOLOGY. Bioinformatics 15:1047–1054

    Article  CAS  PubMed  Google Scholar 

  9. Lefranc M-P, Giudicelli V, Ginestoux C, Bosc N, Folch G, Guiraudou D, Jabado-Michaloud J, Magris S, Scaviner D, Thouvenin V, Combres K, Girod D, Jeanjean S, Protat C, Yousfi Monod M, Duprat E, Kaas Q, Pommié C, Chaume D, Lefranc G (2004) IMGT-ONTOLOGY for immunogenetics and immunoinformatics. In Silico Biol 4:17–29

    CAS  PubMed  Google Scholar 

  10. Lefranc M-P, Clément O, Kaas Q, Duprat E, Chastellan P, Coelho I, Combres K, Ginestoux C, Giudicelli V, Chaume D, Lefranc G (2005) IMGT-Choreography for immunogenetics and immunoinformatics. In Silico Biol 5:45–60

    CAS  PubMed  Google Scholar 

  11. Lefranc M-P, Giudicelli V, Regnier L, Duroux P (2008) IMGT®, a system and an ontology that bridge biological and computational spheres in bioinformatics. Brief Bioinform 9:263–275

    Article  CAS  PubMed  Google Scholar 

  12. Duroux P, Kaas Q, Brochet X, Lane J, Ginestoux C, Lefranc M-P, Giudicelli V (2008) IMGT-Kaleidoscope, the Formal IMGT-ONTOLOGY paradigm. Biochimie 90:570–583

    Article  CAS  PubMed  Google Scholar 

  13. Lefranc M-P (2013) IMGT-ONTOLOGY. In: Dubitzky W, Wolkenhauer O, Yokota H, Cho KH (ed) Encyclopedia of Systems Biology, Springer Science  +  Business Media, Dordrecht, Netherlands doi: 10.1007/978-1-4419-9863-7, LLC012 (in press)

  14. Lefranc M-P (2004) IMGT, the international ImMunoGenetics information system®. In: Lo BKC (ed) Antibody engineering methods and protocols, vol 248, 2nd edn, Methods in molecular biology. Humana Press, Totowa, NJ, USA, pp 27–49

    Google Scholar 

  15. Lefranc M-P (2009) Antibody databases and tools: The IMGT® experience. In: Zhiqiang An (ed) Therapeutic monoclonal antibodies: from Bench to Clinic, John Wiley, 8 Sons, Inc., Hoboker, New Jersey, chap. 4, pp. 91–114.

  16. Ehrenmann F, Duroux P, Giudicelli V, Lefranc M-P (2010) Standardized sequence and structure analysis of antibody using IMGT®, chap. 2. In: Kontermann R, Dübel S (eds) Antibody engineering, vol 2. Springer, Berlin, pp 11–31

    Chapter  Google Scholar 

  17. Lefranc M-P (2009) Antibody databases: IMGT®, a French platform of world-wide interest [in French]. bases de données anticorps: IMGT®, une plate-forme française d’intérêt mondial. Médecine/Sciences 25:1020–1023

    Article  Google Scholar 

  18. Lefranc M-P (2011) Antibody nomenclature: from IMGT-ONTOLOGY to INN definition. MAbs 3(1):1–2

    Article  PubMed Central  PubMed  Google Scholar 

  19. Giudicelli V, Chaume D, Lefranc M-P (2004) IMGT/V-QUEST, an integrated software for immunoglobulin and T cell receptor V-J and V-D-J rearrangement analysis. Nucl Acids Res 32:W435–W440

    Article  CAS  PubMed  Google Scholar 

  20. Brochet X, Lefranc M-P, Giudicelli V (2008) IMGT/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis. Nucl Acids Res 36:W503–W508

    Article  CAS  PubMed  Google Scholar 

  21. Yousfi Monod M, Giudicelli V, Chaume D, Lefranc M-P (2004) IMGT/JunctionAnalysis: the first tool for the analysis of the immunoglobulin and T cell receptor complex V-J and V-D-J JUNCTIONs. Bioinformatics 20:i379–i385

    Article  PubMed  Google Scholar 

  22. Giudicelli V, Lefranc M-P (2005) Interactive IMGT on-line tools for the analysis of immunoglobulin and T cell receptor repertoires. In: Veskler BA (ed) New research on immunology. Nova Science, New York, NY, pp 77–105

    Google Scholar 

  23. Giudicelli V, Lefranc M-P (2008) IMGT® standardized analysis of immunoglobulin rearranged sequences, chapter 2. In: Ghia P, Rosenquist R, Davi F (eds) Immunoglobulin gene analysis in Chronic Lymphocytic Leukemia. Wolters Kluwer Health Italy, Italy, pp 33–52

    Google Scholar 

  24. Giudicelli V, Brochet X, Lefranc M-P (2011) IMGT/V-QUEST: IMGT standardized analysis of the immunoglobulin (IG) and T cell receptor (TR) nucleotide sequences. Cold Spring Harb Protoc 2011(6):695–715. doi:10.1101/pdb.prot5633

    PubMed  Google Scholar 

  25. Giudicelli V, Lefranc M-P (2011) IMGT/JunctionAnalysis: IMGT standardized analysis of the V-J and V-D-J junctions of the rearranged immunoglobulins (IG) and T cell receptors (TR). Cold Spring Harb Protoc 2011(6):716–725. doi:10.1101/pdb.prot5634

    PubMed  Google Scholar 

  26. Alamyar E, Giudicelli V, Duroux P, Lefranc M-P (2010) IMGT/HighV-QUEST: a high-throughput system and web portal for the analysis of rearranged nucleotide sequences of antigen receptors - High-throughput version of IMGT/V-QUEST. Poster, 11èmes Journées Ouvertes de Biologie, Informatique et Mathématiques (JOBIM), Montpellier, 7–9 Sept 2010

    Google Scholar 

  27. Alamyar E, Duroux P, Lefranc M-P, Giudicelli V (2012) IMGT® tools for the nucleotide analysis of immunoglobulin (IG) and T cell receptor (TR) V-(D)-J repertoires, polymorphisms, and IG mutations: IMGT/V-QUEST and IMGT/HighV-QUEST for NGS. In: Christiansen F, Tait B (ed) Immunogenetics. Methods in molecular biology. Humana Press, Springer chap 32, 882:569–604

    Google Scholar 

  28. Alamyar E, Giudicelli V, Li S, Duroux P, Lefranc MP (2012) IMGT/HighV-QUEST: the IMGT® web portal for immunoglobulin (IG) or antibody and T cell receptor (TR) analysis from NGS high throughput and deep sequencing. Immunome Res. 8:26

    Article  CAS  PubMed  Google Scholar 

  29. Giudicelli V, Duroux P, Ginestoux C, Folch G, Jabado-Michaloud J, Chaume D, Lefranc M-P (2006) IMGT/LIGM-DB, the IMGT® comprehensive database of immunoglobulin and T cell receptor nucleotide sequences. Nucl Acids Res 34:D781–D784

    Article  CAS  PubMed  Google Scholar 

  30. Giudicelli V, Chaume D, Lefranc M-P (2005) IMGT/GENE-DB: a comprehensive database for human and mouse immunoglobulin and T cell receptor genes. Nucl Acids Res 33:D256–D261

    Article  CAS  PubMed  Google Scholar 

  31. Ruiz M, Lefranc M-P (2002) IMGT gene identification and Colliers de Perles of human immunoglobulins with known 3D structures. Immunogenetics 53:857–883

    Article  CAS  PubMed  Google Scholar 

  32. Kaas Q, Lefranc M-P (2007) IMGT Colliers de Perles: standardized sequence-structure representations of the IgSF and MhcSF superfamily domains. Current Bioinformatics 2:21–30

    Article  CAS  Google Scholar 

  33. Kaas Q, Ehrenmann F, Lefranc M-P (2007) IG, TR and IgSf, MHC and MhcSF: what do we learn from the IMGT colliers de perles? Brief Funct Genomic Proteomic 6:253–264

    Article  CAS  PubMed  Google Scholar 

  34. Ehrenmann F, Giudicelli V, Duroux P, Lefranc M-P (2011) IMGT/Collier de Perles: IMGT standardized representation of domains (IG, TR, and IgSF variable and constant domains, MH and MhSF groove domains). Cold Spring Harb Protoc 2011(6):726–736. doi:10.1101/pdb.prot5635

    PubMed  Google Scholar 

  35. Kaas Q, Ruiz M, Lefranc M-P (2004) IMGT/3Dstructure-DB and IMGT/StructuralQuery, a database and a tool for immunoglobulin. T cell receptor and MHC structural data Nucl Acids Res 32:D208–D210

    CAS  Google Scholar 

  36. Ehrenmann F, Kaas Q, Lefranc M-P (2010) IMGT/3Dstructure-DB and IMGT/DomainGapAlign: a database and a tool for immunoglobulins or antibodies, T cell receptors, MHC, IgSF and MhcSF. Nucl Acids Res 38:D301–D307

    Article  CAS  PubMed  Google Scholar 

  37. Ehrenmann F, Lefranc M-P (2011) IMGT/3Dstructure-DB: querying the IMGT database for 3D structures in immunology and immunoinformatics (IG or antibodies, TR, MH, RPI, and FPIA). Cold Spring Harb Protoc 2011(6):750–761. doi:10.1101/pdb.prot5637

    PubMed  Google Scholar 

  38. Ehrenmann F, Lefranc M-P (2011) IMGT/DomainGapAlign: IMGT standardized analysis of amino acid sequences of variable, constant, and groove domains (IG, TR, MH, IgSF, MhSF). Cold Spring Harb Protoc 2011(6):737–749. doi:10.1101/pdb.prot5636

    PubMed  Google Scholar 

  39. Lefranc M-P (2011) From IMGT-ONTOLOGY IDENTIFICATION axiom to IMGT standardized keywords: for immunoglobulins (IG), T cell receptors (TR), and conventional genes. Cold Spring Harb Protoc 2011(6):604–613. doi:10.1101/pdb.ip82

    PubMed  Google Scholar 

  40. Lefranc M-P (2011) From IMGT-ONTOLOGY CLASSIFICATION axiom to IMGT standardized gene and allele nomenclature: for immunoglobulins (IG) and T cell receptors (TR). Cold Spring Harb Protoc 2011(6):627–632. doi:10.1101/pdb.ip84

    PubMed  Google Scholar 

  41. Lefranc M-P (2011) From IMGT-ONTOLOGY DESCRIPTION axiom to IMGT standardized labels: for immunoglobulin (IG) and T cell receptor (TR) sequences and structures. Cold Spring Harb Protoc 2011(6):614–626. doi:10.1101/pdb.ip83

    PubMed  Google Scholar 

  42. Lefranc M-P (2011) IMGT unique numbering for the variable (V), constant (C), and groove (G) domains of IG, TR, MH, IgSF, and MhSF. Cold Spring Harb Protoc 2011(6):633–642. doi:10.1101/pdb.ip85

    PubMed  Google Scholar 

  43. Lefranc M-P (2011) IMGT collier de perles for the variable (V), constant (C), and groove (G) domains of IG, TR, MH, IgSF, and MhSF. Cold Spring Harb Protoc 2011(6):643–651. doi:10.1101/pdb.ip86

    PubMed  Google Scholar 

  44. Lefranc M-P (2000) Nomenclature of the human immunoglobulin genes. In: Coligan JE, Bierer BE, Margulies DE, Shevach EM, Strober W (eds) Current protocols in immunology. Wiley, Hoboken N.J., pp A.1P.1–A.1P.37

    Google Scholar 

  45. Lefranc M-P (2000) Nomenclature of the human T cell receptor genes. In: Coligan JE, Bierer BE, Margulies DE, Shevach EM, Strober W (eds) Current protocols in immunology. Wiley, Hoboken N.J., pp A.1O.1–A.1O.23

    Google Scholar 

  46. Wain HM, Bruford EA, Lovering RC, Lush MJ, Wright MW, Povey S (2002) Guidelines for human gene nomenclature. Genomics 79:464–470

    Article  CAS  PubMed  Google Scholar 

  47. Bruford EA, Lush MJ, Wright MW, Sneddon TP, Povey S, Birney E (2008) The HGNC database in 2008: a resource for the human genome. Nucl Acids Res 36:D445–D448

    Article  CAS  PubMed  Google Scholar 

  48. Letovsky SI, Cottingham RW, Porter CJ, Li PW (1998) GDB: the human genome database. Nucl Acids Res 26(1):94–99

    Article  CAS  PubMed  Google Scholar 

  49. Maglott DR, Katz KS, Sicotte H, Pruitt KD (2000) NCBI’s LocusLink and RefSeq. Nucl Acids Res 28(1):126–128

    Article  CAS  PubMed  Google Scholar 

  50. Maglott D, Ostell J, Pruitt KD, Tatusova T (2007) Entrez gene: gene-centered information at NCBI. Nucl Acids Res 35:D26–D31

    Article  CAS  PubMed  Google Scholar 

  51. Stabenau A, McVicker G, Melsopp C, Proctor G, Clamp M, Birney E (2004) The ensembl core software libraries. Genome Res 14:929–933

    Article  CAS  PubMed  Google Scholar 

  52. Wilming LG, Gilbert JG, Howe K, Trevanion S, Hubbard T, Harrow JL (2008) The vertebrate genome annotation (Vega) database. Nucl Acids Res 36:D753–D760

    Article  CAS  PubMed  Google Scholar 

  53. World Health Organization. General policies for monoclonal antibodies. INN Working Document 09.251. Update 18/12/2009, http://www.who.int/medicines/services/inn/en

  54. Lefranc M-P (1997) Unique database numbering system for immunogenetic analysis. Immunol Today 18:509

    Article  CAS  PubMed  Google Scholar 

  55. Lefranc M-P (1999) The IMGT unique numbering for immunoglobulins, T cell receptors and Ig-like domains. The Immunologist 7:132–136

    CAS  Google Scholar 

  56. Lefranc M-P, Pommié C, Ruiz M, Giudicelli V, Foulquier E, Truong L, Thouvenin-Contet V, Lefranc G (2003) IMGT unique numbering for immunoglobulin and T cell receptor variable domains and Ig superfamily V-like domains. Dev Comp Immunol 27:55–77

    Article  CAS  PubMed  Google Scholar 

  57. Lefranc M-P, Pommié C, Kaas Q, Duprat E, Bosc N, Guiraudou D, Jean C, Ruiz M, Da Piedade I, Rouard M, Foulquier E, Thouvenin V, Lefranc G (2005) IMGT unique numbering for immunoglobulin and T cell receptor constant domains and Ig superfamily C-like domains. Dev Comp Immunol 29:185–203

    Article  CAS  PubMed  Google Scholar 

  58. Lefranc M-P, Duprat E, Kaas Q, Tranne M, Thiriot A, Lefranc G (2005) IMGT unique numbering for MHC groove G-DOMAIN and MHC superfamily (MhcSF) G-LIKE-DOMAIN. Dev Comp Immunol 29:917–938

    Article  CAS  PubMed  Google Scholar 

  59. Kabat EA (1991) Sequences of proteins of immunological interest. NIH publication 91–3242, Bethesda, MD

    Google Scholar 

  60. Chothia C, Lesk AM (1987) Canonical structures for the hypervariable regions of immunoglobulins. J Mol Biol 196:901–917

    Article  CAS  PubMed  Google Scholar 

  61. Chothia C, Lesk AM, Tramontano A, Levitt M, Smith-Gill SJ, Air G, Sheriff S, Padlan EA, Davies D, Tulip WR et al (1989) Conformations of immunoglobulin hypervariable regions. Nature 342:877–883

    Article  CAS  PubMed  Google Scholar 

  62. Al-Lazikani B, Lesk AM, Chothia C (1997) Standard conformations for the canonical structures of immunoglobulins. J Mol Biol 273:927–948

    Article  CAS  PubMed  Google Scholar 

  63. Scaviner D, Barbié V, Ruiz M, LefranC M-P (1999) Protein displays of the human immunoglobulin heavy, kappa and lambda variable and joining regions. Exp Clin Immunogenet 16:234–240

    Article  CAS  PubMed  Google Scholar 

  64. Pommié C, Levadoux S, Sabatier R, Lefranc M-P (2004) IMGT standardized criteria for statistical analysis of immunoglobulin V-REGION amino acid properties. J Mol Recognit 17:17–32

    Article  PubMed  Google Scholar 

  65. Rose PW, Beran B, Bi C, Bluhm WF, Dimitropoulos D, Goodsell DS, Prlic A, Quesada M, Quinn GB, Westbrook JD, Young J, Yukich B, Zardecki C, Berman HM, Bourne PE (2011) The RCSB protein data bank: redesigned web site and web services. Nucleic Acids Res 39:D392–D401

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  66. Pelat T, Bedouelle H, Rees AR, Crennell SJ, Lefranc M-P, Thullier P (2008) Germline humanization of a non-human primate antibody that neutralizes the anthrax toxin, by in vitro and in silico engineering. J Mol Biol 2008(384):1400–1407

    Article  Google Scholar 

  67. Magdelaine-Beuzelin C, Kaas Q, Wehbi V, Ohresser M, Jefferis R, Lefranc M-P, Watier H (2007) Structure-function relationships of the variable domains of monoclonal antibodies approved for cancer treatment. Crit Rev Oncol Hematol 64:210–225

    Article  PubMed  Google Scholar 

  68. Stamatopoulos K, Belessi C, Moreno C, Boudjograh M, Guida G, Smilevska T, Belhoul L, Stella S, Stavroyianni N, Crespo M, Hadzidimitriou A, Sutton L, Bosch F, Laoutaris N, Anagnostopoulos A, Montserrat E, Fassas A, Dighiero G, Caligaris-Cappio F, Merle-Béral H, Ghia P, Davi F (2007) Over 20 % of patients with chronic lymphocytic leukemia carry stereotyped receptors: pathogenetic implications and clinical correlations. Blood 109:259–270

    Article  CAS  PubMed  Google Scholar 

  69. Poiron C, Wu Y, Ginestoux C, Ehrenmann F, Duroux P, Lefranc M-P (2010) IMGT/mAb-DB: the IMGT® database for therapeutic monoclonal antibodies. Poster no101, 11èmes Journées Ouvertes de Biologie, Informatique et Mathématiques (JOBIM), Montpellier, 7–9 Sept 2010

    Google Scholar 

  70. Lefranc M-P, Lefranc G Human Gm, Km and Am allotypes and their molecular characterization: a remarkable demonstration of polymorphism. In: Christiansen F, Tait B (ed) Immunogenetics, Methods in molecular biology. Humana Press, Springer chap 34, 882:635–680

    Google Scholar 

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Acknowledgments

We thank Gérard Lefranc for helpful discussions and the IMGT® team for its expertise and constant motivation. IMGT® is a registered trademark of CNRS. IMGT® is a member of the International Medical Informatics Association (IMIA). IMGT® was funded in part by the BIOMED1 (BIOCT930038), Biotechnology BIOTECH2 (BIO4CT960037), fifth PCRDT Quality of Life and Management of Living Resources (QLG2-2000-01287), and sixth PCRDT Information Science and Technology (ImmunoGrid, FP6 IST-028069) programmes of the European Union (EU). IMGT® is currently supported by the Centre National de la Recherche Scientifique (CNRS), the Ministère de l’Enseignement Supérieur et de la Recherche (MESR), the Université Montpellier 2, the GIS IBiSA, the Région Languedoc-Roussillon (Grand Plateau Technique pour la Recherche (GPTR)), the Agence Nationale de la recherche ANR (BIOSYS06_135457, FLAVORES), and the Labex MabImprove.

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  1. Laboratoire d’ImmunoGénétique Moléculaire LIGM, IMGT®, Institut de Génétique Humaine IGH, UPR CNRS 1142, the international ImMunoGeneTics information system®, Montpellier, France

    Marie-Paule Lefranc, François Ehrenmann, Chantal Ginestoux, Véronique Giudicelli & Patrice Duroux

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  1. Marie-Paule Lefranc
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  2. François Ehrenmann
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  3. Chantal Ginestoux
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  4. Véronique Giudicelli
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Correspondence to Marie-Paule Lefranc .

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  1. Antibody Therapeutics & Immunotargeting, INSERM U624, Marseille CEDEX 09, France

    Patrick Chames

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Lefranc, MP., Ehrenmann, F., Ginestoux, C., Giudicelli, V., Duroux, P. (2012). Use of IMGT® Databases and Tools for Antibody Engineering and Humanization. In: Chames, P. (eds) Antibody Engineering. Methods in Molecular Biology, vol 907. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-974-7_1

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