Abstract
Apolipoprotein C3 (ApoC3) is synthesized in liver so that levels or isoform distributions may constitute indicators of liver pathogenesis. The glycoforms of intact protein ApoC3 in serum or plasma can be readily analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry after a one-step extraction using a C4 reverse-phase ZipTip. Glycoform distributions were sensitive to severe systemic diseases such as sepsis or liver diseases such as chronic hepatitis C and alcoholic liver cirrhosis. Glycoisoform distributions were also altered in persons with elevated body mass index and were corrected to normal distribution by metformin, a common drug used for diabetes therapy. This simple method may offer an approach to analysis of health and the mechanism of drug therapies.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kirmiz C, Li B, An HJ, Clowers BH, Chew HK, Lam KS, Ferrige A, Alecio R, Borowsky AD, Sulaimon S, Lebrilla CB, Miyamoto S (2007) A serum glycomics approach to breast cancer biomarkers. Mol Cell Proteomics 6:43–55
Kyselova Z, Mechref Y, Al Bataineh MM, Dobrolecki LE, Hickey RJ, Vinson J, Sweeney CJ, Novotny MV (2007) Alterations in the serum glycome due to metastatic prostate cancer. J Proteome Res 6:1822–1832
Qiu Y, Patwa TH, Xu L, Shedden K, Misek DE, Tuck M, Jin G, Ruffin MT, Turgeon DK, Synal S, Bresalier R, Marcon N, Brenner DE, Lubman DM (2008) Plasma glycoprotein profiling for colorectal cancer biomarker identification by lectin glycoarray and lectin blot. J Proteome Res 7:1693–1703
Blomme B, Van Steenkiste C, Callewaert N, Van Vlierberghe H (2009) Alteration of protein glycosylation in liver diseases. J Hepatol 50:592–603
Harvey SB, Zhang Y, Wilson-Grady J, Monkkonen T, Nelsestuen GL, Kasthuri RS, Verneris MR, Lund TC, Ely EW, Bernard GR, Zeisler H, Homoncik M, Jilma B, Swan T, Kellogg TA (2009) O-glycoside biomarker of apolipoprotein C3: responsiveness to obesity, bariatric surgery, and therapy with metformin, to chronic or severe liver disease and to mortality in severe sepsis and graft vs host disease. J Proteome Res 8:603–612
Ceciliani F, Pocacqua V (2007) The acute phase protein alpha1-acid glycoprotein: a model for altered glycosylation during diseases. Curr Protein Pept Sci 8:91–108
Durand G, Seta N (2000) Protein glycosylation and diseases: blood and urinary oligosaccharides as markers for diagnosis and therapeutic monitoring. Clin Chem 46:795–805
Turnbull JE, Field RA (2007) Emerging glycomics technologies. Nat Chem Biol 3:74–77
Siuti N, Kelleher NL (2007) Decoding protein modifications using top-down mass spectrometry. Nat Methods 4:817–821
Nelsestuen GL, Zhang Y, Martinez MB, Key NS, Jilma B, Verneris M, Sinaiko A, Kasthuri RS (2005) Plasma protein profiling: unique and stable features of individuals. Proteomics 5:4012–4024
Zhang Y, Oetting WS, Harvey SB, Stone MD, Monkkonen T, Matas AJ, Cosio FG, Nelsestuen GL (2009) Urinary Peptide patterns in native kidneys and kidney allografts. Transplantation 87:1807–1813
Akkina SK, Zhang Y, Nelsestuen GL, Oetting WS, Ibrahlm HN (2009) Temporal stability of the urinary proteome after kidney transplant: more sensitive than protein composition? J Proteome Res 8:94–103
Nelsestuen GL, Martinez MB, Hertz MI, Savik K, Wendt CH (2005) Proteomic identification of human neutrophil alpha-defensins in chronic lung allograft rejection. Proteomics 5:1705–1713
Zhang Y, Wroblewski M, Hertz MI, Wendt CH, Cervenka TM, Nelsestuen GL (2006) Analysis of chronic lung transplant rejection by MALDI-TOF profiles of bronchoalveolar lavage fluid. Proteomics 6:1001–1010
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media New York
About this protocol
Cite this protocol
Zhang, Y., Sinaiko, A.R., Nelsestuen, G.L. (2012). Glycoproteins and Glycosylation: Apolipoprotein C3 Glycoforms by Top-Down MALDI-TOF Mass Spectrometry. In: Josic, D., Hixson, D. (eds) Liver Proteomics. Methods in Molecular Biology, vol 909. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-959-4_10
Download citation
DOI: https://doi.org/10.1007/978-1-61779-959-4_10
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-958-7
Online ISBN: 978-1-61779-959-4
eBook Packages: Springer Protocols