Abstract
The native complexity of plant cell walls makes research on them challenging. Hence, it is advantageous to have a diversity of tools that can be used to analyze and characterize plant cell walls. In this chapter, we describe one of two immunological approaches that can be employed for screening of plant cell wall/biomass materials from diverse plants and tissues. This approach, Glycome Profiling, lends itself well to moderate to high-throughput screening of plant cell wall/biomass samples. Glycome Profiling is being further optimized to reduce the amount of sample required for the analysis, and to improve the sensitivity and throughput of the assay. We are optimistic that Glycome Profiling will prove to be a broadly applicable experimental approach that will find increasing application to a wide variety of studies on plant cell wall/biomass samples.
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Acknowledgements
Research in our laboratory on immunological approaches to biomass characterization is supported by the Office of Biological and Environmental Research in the DOE Office of Science through the BioEnergy Science Center (BESC) funded by grant DE-AC05-00OR22725. Generation of the CCRC series of plant glycan-directed mAbs used in this work was supported by the NSF Plant Genome Program (DBI-0421683).
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Pattathil, S., Avci, U., Miller, J.S., Hahn, M.G. (2012). Immunological Approaches to Plant Cell Wall and Biomass Characterization: Glycome Profiling. In: Himmel, M. (eds) Biomass Conversion. Methods in Molecular Biology, vol 908. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-956-3_6
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DOI: https://doi.org/10.1007/978-1-61779-956-3_6
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