Abstract
Detailed protocols for the preparation of CdSe nanocrystals coated with a multishell structure of ZnS and their surface modification based on the successive ion layer adsorption and reaction method are described. The first phase of the synthesis produces a hydrophobic surface consisting of a TOPO/HDA ligand mixture. This is followed by a surface modification of the quantum dots (QDs) with 3-mercaptopropionic acid by the phase transfer method. The modified QDs become soluble in aqueous systems such as water or buffers for further biological applications.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Liu W, Howarth M, Greytak AB, Zheng Y, Nocera DG, Ting AY, Bawendi MG (2008) Compact biocompatible quantum dots functionalized for cellular imaging. J Am Chem Soc 130:1274–1284
Aldana J, Wang YA, Peng X (2001) Photochemical instability of CdSe nanocrystals coated by hydrophilic thiols. J Am Chem Soc 123:8844–8850
Li JJ, Wang A, Guo W, Keay JC, Mishima TD, Johnson MB, Peng X (2003) Large-scale synthesis of nearly monodisperse CdSe/CdS core/shell nanocrystals using air-stable reagents via successive ion layer adsorption and reaction. J Am Chem Soc 125:12567–12575
Xie R, Kolb U, Li J, Bashé T, Mews A (2005) Synthesis and characterization of highly luminescent CdSe-core CdS/Zn0.5Cd0.5S/ZnS multishell nanocrystals. J Am Chem Soc 127:7480–7488
Wang Q, Xu Y, Zhao X, Chang Y, Liu Y, Jiang L, Sharma J, Seo D, Yan H (2007) A facile one-step in situ functionalization of quantum dots with preserved photoluminescence for bioconjugation. J Am Chem Soc 129:6380–6381
Chan WCW, Nie S (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281:2016–2018
Yu WW, Peng X (2002) Formation of high-quality and other II–VI semiconductor nanocrystals in noncoordinating solvents: tunable reactivity of monomers. Angew Chem Int Ed 41:2368–2371
Battaglia D, Peng X (2002) Formation of high quality InP and InAs nanocrystals in a noncoordinating solvent. Nano Lett 2:1027–1030
Peng X, Schlamp MC, Kadavanich AV, Alivisatos AP (1997) Epitaxial growth of highly luminescent CdSe/CdS core/shell nanocrystals with photostability and electronic accessibility. J Am Chem Soc 119:7019–7029
Gill R, Freeman R, Xu J, Willner I, Winograd S, Shweky I, Banin U (2006) Probing biocatalytic transformations with CdSe-ZnS QDs. J Am Chem Soc 128:15376–15377
Williams ATR, Winfield SA, Miller JN (1983) Relative fluorescence quantum yields using a computer-controlled luminescence spectrometer. Analyst 108:1067–1071
Yu WW, Chang E, Falkner JC, Zhang J, Al-Somali AM, Sayes CM, Johns J, Drezek R, Colvin VL (2007) Forming biocompatible nonaggregated nanocrystals in water using amphiphilic polymers. J Am Chem Soc 129:2871–2879
Yu WW, Qu L, Guo W, Peng X (2003) Experimental determination of the extinction coefficient of CdTe, CdSe and CdS nanocrystals. Chem Mater 15:2854–2860
Leatherdale CA, Woo WK, Mikulec FV, Bawendi MG (2002) On the absorption cross section of CdSe nanocrystal quantum dots. J Phys Chem B 106:7619–7622
Striolo A, Ward J, Prausnitz JM, Parak WJ, Zanchet D, Gerion D, Milliron D, Alivisatos AP (2002) Molecular weight, osmotic second virial coefficient and extinction coefficient of colloidal CdSe nanocrystals. J Phys Chem B 106:5500–5505
Acknowledgements
This work was supported by a grant to A.S.M., the grant #PEST-OE/EQB/LA004/2011 and the project NTec/SQA/0131/2007 from the Fundação para a Ciência e Tecnologia.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Miguel, A.S., Maycock, C., Oliva, A. (2012). Synthesis and Functionalization of CdSe/ZnS QDs Using the Successive Ion Layer Adsorption Reaction and Mercaptopropionic Acid Phase Transfer Methods. In: Soloviev, M. (eds) Nanoparticles in Biology and Medicine. Methods in Molecular Biology, vol 906. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-953-2_10
Download citation
DOI: https://doi.org/10.1007/978-1-61779-953-2_10
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-952-5
Online ISBN: 978-1-61779-953-2
eBook Packages: Springer Protocols