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Guidelines for High-Resolution Genotyping of Chlamydia trachomatis Using Multilocus Sequence Analysis

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Diagnosis of Sexually Transmitted Diseases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 903))

Abstract

Chlamydia is one of the most common sexually transmitted infections worldwide and can cause ectopic pregnancies and infertility. It is therefore important to have adequate genotyping tools for investigating the spread of Chlamydia trachomatis among the population. Here, we describe a high-resolution multilocus sequence typing (MLST) system able to differentiate closely related clinical strains, which makes it ideal for short-term epidemiology and outbreak investigations. It is based on five highly variable but stable target regions which are PCR amplified and DNA sequenced.

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References

  1. Pedersen LN, Herrmann B, Moller JK (2009) Typing Chlamydia trachomatis: from egg yolk to nanotechnology. FEMS Immunol Med Microbiol 55:120–130

    Article  PubMed  CAS  Google Scholar 

  2. van Belkum A, Tassios PT, Dijkshoorn L, Haeggman S, Cookson B, Fry NK, Fussing V, Green J, Feil E, Gerner-Smidt P, Brisse S, Struelens M (2007) Guidelines for the validation and application of typing methods for use in bacterial epidemiology. Clin Microbiol Infect 13(Suppl 3):1–46

    Article  PubMed  Google Scholar 

  3. Pannekoek Y, Morelli G, Kusecek B, Morre SA, Ossewaarde JM, Langerak AA, van der Ende A (2008) Multi locus sequence typing of Chlamydiales: clonal groupings within the obligate intracellular bacteria Chlamydia trachomatis. BMC Microbiol 8:42

    Article  PubMed  Google Scholar 

  4. Dean D, Bruno WJ, Wan R, Gomes JP, Devignot S, Mehari T, de Vries HJ, Morre SA, Myers G, Read TD, Spratt BG (2009) Predicting phenotype and emerging strains among Chlamydia trachomatis infections. Emerg Infect Dis 15:1385–1394

    Article  PubMed  CAS  Google Scholar 

  5. Klint M, Fuxelius HH, Goldkuhl RR, Skarin H, Rutemark C, Andersson SG, Persson K, Herrmann B (2007) High-resolution genotyping of Chlamydia trachomatis strains by multilocus sequence analysis. J Clin Microbiol 45:1410–1414

    Article  PubMed  CAS  Google Scholar 

  6. Pedersen LN, Podenphant L, Moller JK (2008) Highly discriminative genotyping of Chlamydia trachomatis using omp1 and a set of variable number tandem repeats. Clin Microbiol Infect 14:644–652

    Article  PubMed  CAS  Google Scholar 

  7. Ikryannikova LN, Shkarupeta MM, Shitikov EA, Il’ina EN, Govorun VM (2010) Comparative evaluation of new typing schemes for urogenital Chlamydia trachomatis isolates. FEMS Immunol Med Microbiol 59:188–196

    PubMed  CAS  Google Scholar 

  8. Jurstrand M, Christerson L, Klint M, Fredlund H, Unemo M, Herrmann B (2010) Characterisation of Chlamydia trachomatis by ompA sequencing and multilocus sequence typing in a Swedish county before and after identification of the new variant. Sex Transm Infect 86:56–60

    Article  PubMed  Google Scholar 

  9. Harding-Esch EM, Christerson L, Grannas K, Roberts CH, Holland MJ, Andreasen AA, Sillah A, Sarr B, Herrmann B, Bailey RL, Mabey DC (2010) Multi-locus sequence typing: a useful tool for trachoma molecular epidemiology. In: Proceedings of the 12th international symposium on human chlamydial infections, pp 55–58

    Google Scholar 

  10. Herrmann B, Torner A, Low N, Klint M, Nilsson A, Velicko I, Soderblom T, Blaxhult A (2008) Emergence and spread of Chlamydia trachomatis variant. Sweden, Emerg Infect Dis 14:1462–1465

    Article  Google Scholar 

  11. Klint M, Hadad R, Christerson L, Lore B, Anagrius C, Osterlund A, Larsson I, Sylvan S, Fredlund H, Unemo M, Herrmann B (2011) Prevalence trends in Sweden of the new variant of Chlamydia trachomatis. Clin Microbiol Infect 17:683–689

    Article  PubMed  CAS  Google Scholar 

  12. Christerson L, de Vries HJ, Klint M, Herrmann B, Morre SA (2011) Multilocus sequence typing of urogenital Chlamydia trachomatis from patients with different degrees of clinical symptoms. Sex Transm Dis 38:490–494

    PubMed  Google Scholar 

  13. Gravningen K, Christerson L, Ståhlsten A, Simonsen G, Ödman K, Furberg AS, Herrmann B (2011) A population study on Chlamydia trachomatis in adolescents in a high incidence area in North Norway reveals multiple new MLST genotypes and the new Swedish mutant (nvCT). In: 21st European congress of clinical microbiology and infectious diseases, P1734

    Google Scholar 

  14. Christerson L, de Vries HJ, de Barbeyrac B, Gaydos CA, Henrich B, Hoffmann S, Schachter J, Thorvaldsen J, Vall-Mayans M, Klint M, Herrmann B, Morre SA (2010) Typing of lymphogranuloma venereum Chlamydia trachomatis strains. Emerg Infect Dis 16:1777–1779

    Article  PubMed  Google Scholar 

  15. Chen CY, Chi KH, Alexander S, Ison CA, Ballard RC (2008) A real-time quadriplex PCR assay for the diagnosis of rectal lymphogranuloma venereum and non-lymphogranuloma venereum Chlamydia trachomatis infections. Sex Transm Infect 84:273–276

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden

    Linus Christerson & Björn Herrmann

Authors
  1. Linus Christerson
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  2. Björn Herrmann
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Corresponding author

Correspondence to Björn Herrmann .

Editor information

Editors and Affiliations

  1. University Hospital, Institute of Medical Microbiology, Heinrich-Heine-University, Universitätssraße 1, Düsseldorf, 40225, Germany

    Colin R. MacKenzie

  2. University Hospital, Institute of Medical Microbiology, Heinrich-Heine-University, Universitätssraße 1, Düsseldorf, 40225, Germany

    Birgit Henrich

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© 2012 Springer Science+Business Media New York

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Christerson, L., Herrmann, B. (2012). Guidelines for High-Resolution Genotyping of Chlamydia trachomatis Using Multilocus Sequence Analysis. In: MacKenzie, C., Henrich, B. (eds) Diagnosis of Sexually Transmitted Diseases. Methods in Molecular Biology, vol 903. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-937-2_4

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  • DOI: https://doi.org/10.1007/978-1-61779-937-2_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-936-5

  • Online ISBN: 978-1-61779-937-2

  • eBook Packages: Springer Protocols

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