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Fluorescence Correlation Spectroscopy to Determine the Diffusion Coefficient of α-Synuclein and Follow Early Oligomer Formation

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Intrinsically Disordered Protein Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 895))

Abstract

Fluorescence correlation spectroscopy (FCS) can be used to determine the diffusion coefficient of fluorescently labeled α-synuclein. It is a technique based on the use of a confocal microscope. By applying FCS in a combination of short sampling times and repeated measurements, the disappearance of individual α-synuclein molecules (called monomers) and the formation of oligomers can be characterized during the early aggregation process.

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Authors and Affiliations

  1. Laboratory of Biomolecular Dynamics, University of Leuven, Leuven, Belgium

    Sangeeta Nath, Manli Deng & Yves Engelborghs

Authors
  1. Sangeeta Nath
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  2. Manli Deng
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  3. Yves Engelborghs
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Corresponding author

Correspondence to Yves Engelborghs .

Editor information

Editors and Affiliations

  1. Department of Molecular Medicine, College of Medicine, University of South Florida, Bruce B. Downs Blvd 12901, Tampa, 33612, Florida, USA

    Vladimir N. Uversky

  2. Dept. Biochemistry & Molecular Biology, Center for Computational Biology &, Indiana University, West 10th Street 410, Indianapolis, 46202, Indiana, USA

    A. Keith Dunker

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Nath, S., Deng, M., Engelborghs, Y. (2012). Fluorescence Correlation Spectroscopy to Determine the Diffusion Coefficient of α-Synuclein and Follow Early Oligomer Formation. In: Uversky, V., Dunker, A. (eds) Intrinsically Disordered Protein Analysis. Methods in Molecular Biology, vol 895. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-927-3_29

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  • DOI: https://doi.org/10.1007/978-1-61779-927-3_29

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-926-6

  • Online ISBN: 978-1-61779-927-3

  • eBook Packages: Springer Protocols

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