Abstract
This chapter presents three methods for re-constructing mouse foetal kidney tissue from simple suspensions of cells. These techniques are very useful for a number of purposes: (1) they allow the production of fine-grained chimaeras in which cell autonomy of mutations can be tested, (2) they provide an environment that allows the renal differentiation potential of stem cells to be assessed, and (3) they are an excellent system in which to study the mechanisms of self-organization. Each of the methods described here begins with disaggregation of embryonic mouse kidneys, followed by re-aggregation and culture; the main differences are in the culture methods, each of which has advantages for particular purposes.
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References
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Acknowledgements
This work was supported by NC3Rs grant G0700480 and EU Star-t-Rek network FP7 223007 grants to J.A.D. and Australian Stem Cell Centre P067 and National Health and Medical Research Council of Australia ID631362 grants to M.H.L. M.H.L. is a Principal Research Fellow of the NHMRC. We thank Caroline Hendry, Institute for Molecular Bioscience, for assistance with Figures.
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Davies, J.A., Unbekandt, M., Ineson, J., Lusis, M., Little, M.H. (2012). Dissociation of Embryonic Kidney Followed by Re-aggregation as a Method for Chimeric Analysis. In: Michos, O. (eds) Kidney Development. Methods in Molecular Biology™, vol 886. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-851-1_12
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DOI: https://doi.org/10.1007/978-1-61779-851-1_12
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