Abstract
Exposure of the zebrafish retina to intense light is a noninvasive method to elicit the selective degeneration of photoreceptors. In zebrafish, photoreceptor degeneration is followed by robust photoreceptor regeneration from stem cells that are intrinsic to the teleost retina. Two recent light-lesioning methods have been developed, each with specific advantages. The first involves a prolonged period of dark adaptation followed by exposure to high-intensity halogen lighting at ∼3,000–20,000 lux for 3–4 days. This causes widespread degeneration of rod and cone cells in the dorsal and central regions of the retina. The second method uses ultrahigh-intensity lighting at intensities greater than 120,000 lux, with an exposure time of 30 min. This causes degeneration of rod and cone cells within a relatively narrow naso-temporal band in the central retina. The advantages of the first (lower light intensity) technique are the widespread destruction of photoreceptors and the lower cost of equipment. The advantages of the second (higher light intensity) technique are the elimination of the prolonged dark adaptation and short duration of the exposure, thereby allowing experiments to be conducted more rapidly.
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References
Craig SEL, Calinescu A-A, Hitchcock P (2008) Identification of the molecular signatures integral to regenerating photoreceptors in the retina of the zebra fish. J Ocul Biol Dis Inform 1:73–84
Liu Q (2002) Up-regulation of cadherin-2 and cadherin-4 in regenerating visual structures of adult zebrafish. Exp Neurol 177:396–406
Senut MC, Gulati-Leekha A, Goldman D (2004) An element in the alpha1-tubulin promoter is necessary for retinal expression during optic nerve regeneration but not after eye injury in the adult zebrafish. J Neurosci Off J Soc Neurosci 24:7663–7673
Fimbel SM et al (2007) Regeneration of inner retinal neurons after intravitreal injection of ouabain in zebrafish. J Neurosci Off J Soc Neurosci 27:1712–1724
Wu DM et al (2001) Cones regenerate from retinal stem cells sequestered in the inner nuclear layer of adult goldfish retina. Invest Ophthalmol Vis Sci 42:2115–2124
Raymond P et al (2006) Molecular characterization of retinal stem cells and their niches in adult zebrafish. BMC Dev Biol 6:36
Morris A et al (2005) Cone survival despite rod degeneration in XOPS-mCFP transgenic zebrafish. Invest Ophthalmol Vis Sci 46:4762–4771
Vihtelic T, Hyde D (2000) Light-induced rod and cone cell death and regeneration in the adult albino zebrafish (Danio rerio) retina. J Neurobiol 44:289–307
Vihtelic T et al (2006) Retinal regional differences in photoreceptor cell death and regeneration in light-lesioned albino zebrafish. Exp Eye Res 82:558–575
Bernardos R et al (2007) Late-stage neuronal progenitors in the retina are radial Müller glia that function as retinal stem cells. J Neurosci Off J Soc Neurosci 27:7028–7040
Lawrence C (2007) The husbandry of zebrafish (Danio rerio): a review. Aquaculture 269:1–20
Craig SE et al (2010) The zebrafish galectin Drgal1-l2 is expressed by proliferating Müller glia and photoreceptor progenitors and regulates the regeneration of rod photoreceptors. Invest Ophthalmol Vis Sci 51:3244–3252
Kassen SC et al (2008) The Tg(ccnb1:EGFP) transgenic zebrafish line labels proliferating cells during retinal development and regeneration. Mol Vis 14:951–963
Calinescu A et al (2009) Cellular expression of Midkine-a and Midkine-b during retinal development and photoreceptor regeneration in zebrafish. J Comp Neurol 514:1–10
Kassen S et al (2007) Time course analysis of gene expression during light-induced photoreceptor cell death and regeneration in albino zebrafish. Dev Neurobiol 67:1009–1031
Thummel R et al (2008) Inhibition of Müller glial cell division blocks regeneration of the light-damaged zebrafish retina. Dev Neurobiol 68:392–408
Thummel R et al (2008) Characterization of Müller glia and neuronal progenitors during adult zebrafish retinal regeneration. Exp Eye Res 87:433–444
Thummel R et al (2010) Pax6a and Pax6b are required at different points in neuronal progenitor cell proliferation during zebrafish photoreceptor regeneration. Exp Eye Res 90:572–582
Thomas J et al (2012) Characterization of multiple light damage paradigms reveals regional differences in photoreceptor loss. Exp Eye Res 97:105–116
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Taylor, S., Chen, J., Luo, J., Hitchcock, P. (2012). Light-Induced Photoreceptor Degeneration in the Retina of the Zebrafish. In: Wang, SZ. (eds) Retinal Development. Methods in Molecular Biology, vol 884. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-848-1_17
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DOI: https://doi.org/10.1007/978-1-61779-848-1_17
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