Skip to main content
SpringerLink
Log in

Promoter Methylation in Head and Neck Tumorigenesis

  • Protocol
  • First Online:
Cancer Epigenetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 863))

Abstract

In addition to genetic alterations of gains and losses, epigenetic events of promoter methylation appear to further undermine a destabilized genomic repertoire in squamous head and neck carcinoma (HNSCC). This chapter provides an overview of frequently methylated tumor suppressor genes in benign head and neck papillomas, primary HNSCC tumors, and HNSCC cell lines and their relevance as epigenetic markers in head and neck tumorigenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Jemal, A., Tiwari, R.C., Murray, T., Ghafoor, A., Samuels, A., Ward, E., et al. (2004) Cancer statistics, 2004. CA Cancer J Clin 54, 8–29.

    Article  PubMed  Google Scholar 

  2. Costello, J.F.and Plass, C. (2001) Methylation matters. J Med Genet 38, 285–303.

    Article  PubMed  CAS  Google Scholar 

  3. Li, E., Bestor, T.H., and Jaenisch, R. (1992) Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell 69, 915–926.

    Article  PubMed  CAS  Google Scholar 

  4. Li, E., Beard, C., and Jaenisch, R. (1993) Role for DNA methylation in genomic imprinting. Nature 366, 362–365.

    Article  PubMed  CAS  Google Scholar 

  5. Pfeifer, G.P., Tanguay, R.L., Steigerwald, S.D., and Riggs, A.D. (1990) In vivo footprint and methylation analysis by PCR-aided genomic sequencing: comparison of active and inactive X chromosomal DNA at the CpG island and promoter of human PGK-1. Genes Dev 4, 1277–1287.

    Article  PubMed  CAS  Google Scholar 

  6. Costello, J.F., Fruhwald, M.C., Smiraglia, D.J., Rush, L.J., Robertson, G.P., Gao, X., et al. (2000) Aberrant CpG-island methylation has non-random and tumour-type-specific patterns. Nat Genet 24, 132–138.

    Article  PubMed  CAS  Google Scholar 

  7. Issa, J.P., Vertino, P.M., Wu, J., Sazawal, S., Celano, P., Nelkin, B.D., et al. (1993) Increased cytosine DNA-methyltransferase activity during colon cancer progression. J Natl Cancer Inst 85, 1235–1240.

    Article  PubMed  CAS  Google Scholar 

  8. Lin, S.Y., Yeh, K.T., Chen, W.T., Chen, H.C., Chen, S.T., and Chang, J.G. (2004) Promoter CpG methylation of caveolin-1 in sporadic colorectal cancer. Anticancer Res 24, 1645–1650.

    PubMed  CAS  Google Scholar 

  9. Smiraglia, D.J., Smith, L.T., Lang, J.C., Rush, L.J., Dai, Z., Schuller, D.E., et al. (2003) Differential targets of CpG island hypermethylation in primary and metastatic head and neck squamous cell carcinoma (HNSCC). J Med Genet 40, 25–33.

    Article  PubMed  CAS  Google Scholar 

  10. Baylin, S.B., Herman, J.G., Graff, J.R., Vertino, P.M., and Issa, J.P. (1998) Alterations in DNA methylation: a fundamental aspect of neoplasia. Adv Cancer Res 72, 141–196.

    Article  PubMed  CAS  Google Scholar 

  11. Jones, P.A. and Laird, P.W. (1999) Cancer epigenetics comes of age. Nat Genet 21, 163–167.

    Article  PubMed  CAS  Google Scholar 

  12. Chan, M.F., Liang, G., and Jones, P.A. (2000) Relationship between transcription and DNA methylation. Curr Top Microbiol Immunol 249, 75–86.

    Article  PubMed  CAS  Google Scholar 

  13. Cairns, P. (2004) Detection of promoter hypermethylation of tumor suppressor genes in urine from kidney cancer patients. Ann N Y Acad Sci 1022, 40–43.

    Article  PubMed  CAS  Google Scholar 

  14. Kim, H., Kwon, Y.M., Kim, J.S., Lee, H., Park, J.H., Shim, Y.M., et al. (2004) Tumor-specific methylation in bronchial lavage for the early detection of non-small-cell lung cancer. J Clin Oncol 22, 2363–2370.

    Article  PubMed  CAS  Google Scholar 

  15. Roman-Gomez, J., Jimenez-Velasco, A., Castillejo, J.A., Agirre, X., Barrios, M., Navarro, G., et al. (2004) Promoter hypermethylation of cancer-related genes: a strong independent prognostic factor in acute lymphoblastic leukemia. Blood 104, 2492–2498.

    Article  PubMed  CAS  Google Scholar 

  16. Sanz-Ortega, J., Saez, M.C., Sierra, E., Torres, A., Balibrea, J.L., Hernando, F., et al. (2001) 3p21, 5q21, and 9p21 allelic deletions are frequently found in normal bronchial cells adjacent to non-small-cell lung cancer, while they are unusual in patients with no evidence of malignancy. J Pathol 195, 429–434.

    Article  PubMed  CAS  Google Scholar 

  17. Yoo, W.J., Cho, S.H., Lee, Y.S., Park, G.S., Kim, M.S., Kim, B.K., et al. (2004) Loss of heterozygosity on chromosomes 3p,8p,9p and 17p in the progression of squamous cell carcinoma of the larynx. J Korean Med Sci 19, 345–351.

    Article  PubMed  CAS  Google Scholar 

  18. Sanz-Ortega, J., Valor, C., Saez, M.C., Ortega, L., Sierra, E., Poch, J., et al. (2003) 3p21, 5q21, 9p21 and 17p13 allelic deletions accumulate in the dysplastic spectrum of laryngeal carcinogenesis and precede malignant transformation. Histol Histopathol 18, 1053–1057.

    PubMed  CAS  Google Scholar 

  19. Papadimitrakopoulou, V.A., Izzo, J., Mao, L., Keck, J., Hamilton, D., Shin, D.M., et al. (2001) Cyclin D1 and p16 alterations in advanced premalignant lesions of the upper aerodigestive tract: role in response to chemoprevention and cancer development. Clin Cancer Res 7, 3127–3134.

    PubMed  CAS  Google Scholar 

  20. Emilion, G., Langdon, J.D., Speight, P., and Partridge, M. (1996) Frequent gene deletions in potentially malignant oral lesions. Br J Cancer 73, 809–813.

    Article  PubMed  CAS  Google Scholar 

  21. Roz, L., Wu, C.L., Porter, S., Scully, C., Speight, P., Read, A., et al. (1996) Allelic imbalance on chromosome 3p in oral dysplastic lesions: an early event in oral carcinogenesis. Cancer Res 56, 1228–1231.

    PubMed  CAS  Google Scholar 

  22. Miracca, E.C., Kowalski, L.P., and Nagai, M.A. (1999) High prevalence of p16 genetic alterations in head and neck tumours. Br J Cancer 81, 677–683.

    Article  PubMed  CAS  Google Scholar 

  23. Esteller, M., Corn, P.G., Baylin, S.B., and Herman, J.G. (2001) A gene hypermethylation profile of human cancer. Cancer Res 61, 3225–3229.

    PubMed  CAS  Google Scholar 

  24. Rosas, S.L., Koch, W., da Costa Carvalho, M.G., Wu, L., Califano, J., Westra, W., et al. (2001) Promoter hypermethylation patterns of p16, O6-methylguanine-DNA-methyltransferase, and death-associated protein kinase in tumors and saliva of head and neck cancer patients. Cancer Res 61, 939–942.

    PubMed  CAS  Google Scholar 

  25. Hasegawa, M., Nelson, H.H., Peters, E., Ringstrom, E., Posner, M., and Kelsey, K.T. (2002) Patterns of gene promoter methylation in squamous cell cancer of the head and neck. Oncogene 21, 4231–4236.

    Article  PubMed  CAS  Google Scholar 

  26. Viswanathan, M., Tsuchida, N., and Shanmugam, G. (2003) Promoter hypermethylation profile of tumor-associated genes p16, p15, hMLH1, MGMT and E-cadherin in oral squamous cell carcinoma. Int J Cancer 105, 41–46.

    Article  PubMed  CAS  Google Scholar 

  27. El-Naggar, A.K., Lai, S., Clayman, G., Lee, J.K., Luna, M.A., Goepfert, H., et al. (1997) Methylation, a major mechanism of p16/CDKN2 gene inactivation in head and neck squamous carcinoma. Am J Pathol 151, 1767–1774.

    PubMed  CAS  Google Scholar 

  28. Sanchez-Cespedes, M., Esteller, M., Wu, L., Nawroz-Danish, H., Yoo, G.H., Koch, W.M., et al. (2000) Gene promoter hypermethylation in tumors and serum of head and neck cancer patients. Cancer Res 60, 892–895.

    PubMed  CAS  Google Scholar 

  29. Chen, K., Sawhney, R., Khan, M., Benninger, M.S., Hou, Z., Sethi, S., et al. (2007) Methylation of multiple genes as diagnostic and therapeutic markers in primary head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 133, 1131–1138.

    Article  PubMed  Google Scholar 

  30. Zou, C.P., Youssef, E.M., Zou, C.C., Carey, T.E., and Lotan, R. (2001) Differential effects of chromosome 3p deletion on the expression of the putative tumor suppressor RAR beta and on retinoid resistance in human squamous carcinoma cells. Oncogene 20, 6820–6827.

    Article  PubMed  CAS  Google Scholar 

  31. Xu, X.C., Ro, J.Y., Lee, J.S., Shin, D.M., Hong, W.K., and Lotan, R. (1994) Differential expression of nuclear retinoid receptors in normal, premalignant, and malignant head and neck tissues. Cancer Res 54, 3580–3587.

    PubMed  CAS  Google Scholar 

  32. Pegg, A.E. (1990) Mammalian O6-alkylguanine-DNA alkyltransferase: regulation and importance in response to alkylating carcinogenic and therapeutic agents. Cancer Res 50, 6119-6129.

    PubMed  CAS  Google Scholar 

  33. Hirohashi, S. (1998) Inactivation of the E-cadherin-mediated cell adhesion system in human cancers. Am J Pathol 153, 333–339.

    Article  PubMed  CAS  Google Scholar 

  34. Pollack, J.R., Perou, C.M., Alizadeh, A.A., Eisen, M.B., Pergamenschikov, A., Williams, C.F., et al. (1999) Genome-wide analysis of DNA copy-number changes using cDNA microarrays. Nat Genet 23, 41–46.

    Article  PubMed  CAS  Google Scholar 

  35. Khan, J., Saal, L.H., Bittner, M.L., Chen, Y., Trent, J.M., and Meltzer, P.S. (1999) Expression profiling in cancer using cDNA microarrays. Electrophoresis 20, 223–229.

    Article  PubMed  CAS  Google Scholar 

  36. Hanash, S.M., and Teichroew, D. (1998) Mining the human proteome: experience with the human lymphoid protein database. Electrophoresis 19, 2004–2009.

    Article  PubMed  CAS  Google Scholar 

  37. Soltys, S.G., Le, Q.T., Shi, G., Tibshirani, R., Giaccia, A.J., and Koong, A.C. (2004) The use of plasma surface-enhanced laser desorption/ionization time-of-flight mass spectrometry proteomic patterns for detection of head and neck squamous cell cancers. Clin Cancer Res 10, 4806–4812.

    Article  PubMed  CAS  Google Scholar 

  38. Smeets, S.J., Braakhuis, B.J., Abbas, S., Snijders, P.J., Ylstra, B., van de Wiel, M.A., et al. (2006) Genome-wide DNA copy number alterations in head and neck squamous cell carcinomas with or without oncogene-expressing human papillomavirus. Oncogene 25, 2558–2564.

    Article  PubMed  CAS  Google Scholar 

  39. Worsham, M.J., Pals, G., Schouten, J.P., Van Spaendonk, R.M., Concus, A., Carey, T.E., et al. (2003) Delineating genetic pathways of disease progression in head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 129, 702–708.

    Article  PubMed  Google Scholar 

  40. Worsham, M.J., Chen, K.M., Tiwari, N., Pals, G., Schouten, J.P., Sethi, S., et al. (2006) Fine-mapping loss of gene architecture at the CDKN2B (p15INK4b), CDKN2A (p14ARF, p16INK4a), and MTAP genes in head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 132, 409–415.

    Article  PubMed  Google Scholar 

  41. Shaw, R.J., Liloglou, T., Rogers, S.N., Brown, J.S., Vaughan, E.D., Lowe, D., et al. (2006) Promoter methylation of P16, RARbeta, E-cadherin, cyclin A1 and cytoglobin in oral cancer: quantitative evaluation using pyrosequencing. Br J Cancer 94, 561–568.

    Article  PubMed  CAS  Google Scholar 

  42. Sanchez-Cespedes, M., Okami, K., Cairns, P., and Sidransky, D. (2000) Molecular analysis of the candidate tumor suppressor gene ING1 in human head and neck tumors with 13q deletions. Genes Chromosomes Cancer 27, 319–322.

    Article  PubMed  CAS  Google Scholar 

  43. Shah, S.I., Yip, L., Greenberg, B., Califano, J.A., Chow, J., Eisenberger, C.F., et al. (2000) Two distinct regions of loss on chromosome arm 4q in primary head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 126, 1073–1076.

    PubMed  CAS  Google Scholar 

  44. Sidransky, D. (2000) Circulating DNA. What we know and what we need to learn. Ann N Y Acad Sci 906, 1–4.

    Article  CAS  Google Scholar 

  45. Maruya, S., Issa, J.P., Weber, R.S., Rosenthal, D.I., Haviland, J.C., Lotan, R., et al. (2004) Differential methylation status of tumor-associated genes in head and neck squamous carcinoma: incidence and potential implications. Clin Cancer Res 10, 3825–3830.

    Article  PubMed  CAS  Google Scholar 

  46. Worsham, M.J., Chen, K.M., Meduri, V., Nygren, A.O., Errami, A., Schouten, J.P., et al. (2006) Epigenetic events of disease progression in head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 132, 668–677.

    Article  PubMed  Google Scholar 

  47. Stephen, J.K., Vaught, L.E., Chen, K.M., Sethi, S., Shah, V., Benninger, M.S., et al. (2007) Epigenetic events underlie the pathogenesis of sinonasal papillomas. Mod Pathol.

    Google Scholar 

  48. Stephen, J.K., Vaught, L.E., Chen, K.M., Shah, V., Schweitzer, V.G., Gardner, G., et al. (2007) An epigenetically derived monoclonal origin for recurrent respiratory papillomatosis. Arch Otolaryngol Head Neck Surg 133, 684–692.

    Article  PubMed  Google Scholar 

  49. Herman, J.G., Graff, J.R., Myohanen, S., Nelkin, B.D., and Baylin, S.B. (1996) Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci U S A 93, 9821–9826.

    Article  PubMed  CAS  Google Scholar 

  50. Huang, T.H., Perry, M.R., and Laux, D.E. (1999) Methylation profiling of CpG islands in human breast cancer cells. Hum Mol Genet 8, 459–470.

    Article  PubMed  CAS  Google Scholar 

  51. Nygren, A.O., Ameziane, N., Duarte, H.M., Vijzelaar, R.N., Waisfisz, Q., Hess, C.J. et al. (2005) Methylation-specific MLPA (MS-MLPA): simultaneous detection of CpG methylation and copy number changes of up to 40 sequences. Nucleic Acids Res 33, e128.

    Article  PubMed  Google Scholar 

  52. Wan, H., Oridate, N., Lotan, D., Hong, W.K., and Lotan, R. (1999) Overexpression of retinoic acid receptor beta in head and neck squamous cell carcinoma cells increases their sensitivity to retinoid-induced suppression of squamous differentiation by retinoids. Cancer Res 59, 3518–3526.

    PubMed  CAS  Google Scholar 

  53. Youssef, E.M., Lotan, D., Issa, J.P., Wakasa, K., Fan, Y.H., Mao, L., et al. (2004) Hypermethylation of the retinoic acid receptor-beta(2) gene in head and neck carcinogenesis. Clin Cancer Res 10, 1733–1742.

    Article  PubMed  CAS  Google Scholar 

  54. Toyota, M., Sasaki, Y., Satoh, A., Ogi, K., Kikuchi, T., Suzuki, H., et al. (2003) Epigenetic inactivation of CHFR in human tumors. Proc Natl Acad Sci U S A 100, 7818–7823.

    Article  PubMed  CAS  Google Scholar 

  55. Uesugi, H., Uzawa, K., Kawasaki, K., Shimada, K., Moriya, T., Tada, A., et al. (2005) Status of reduced expression and hypermethylation of the APC tumor suppressor gene in human oral squamous cell carcinoma. Int J Mol Med 15, 597–602.

    PubMed  CAS  Google Scholar 

  56. Eads, C.A., Lord, R.V., Kurumboor, S.K., Wickramasinghe, K., Skinner, M.L., Long, T.I. et al. (2000) Fields of aberrant CpG island hypermethylation in Barrett’s esophagus and associated adenocarcinoma. Cancer Res 60, 5021–5026.

    PubMed  CAS  Google Scholar 

  57. Capper, J.W., Bailey, C.M., and Michaels, L. (1983) Squamous papillomas of the larynx in adults. A review of 63 cases. Clin Otolaryngol Allied Sci 8, 109–119.

    Article  PubMed  CAS  Google Scholar 

  58. Bauman, N.M.and Smith, R.J. (1996) Recurrent respiratory papillomatosis. Pediatr Clin North Am 43, 1385–1401.

    Article  PubMed  CAS  Google Scholar 

  59. Batsakis, J.G.and Suarez, P. (2001) Schneiderian papillomas and carcinomas: a review. Adv Anat Pathol 8, 53–64.

    Article  PubMed  CAS  Google Scholar 

  60. Doyle, D.J., Henderson, L.A., LeJeune, F.E., Jr., and Miller, R.H. (1994) Changes in human papillomavirus typing of recurrent respiratory papillomatosis progressing to malignant neoplasm. Arch Otolaryngol Head Neck Surg 120, 1273–1276.

    Article  PubMed  CAS  Google Scholar 

  61. Buchwald, C., Franzmann, M.B., Jacobsen, G.K., and Lindeberg, H. (1995) Human papillomavirus (HPV) in sinonasal papillomas: a study of 78 cases using in situ hybridization and polymerase chain reaction. Laryngoscope 105, 66–71.

    Article  PubMed  CAS  Google Scholar 

  62. Brandwein, M., Steinberg, B., Thung, S., Biller, H., Dilorenzo, T., and Galli, R. (1989) Human papillomavirus 6/11 and 16/18 in Schneiderian inverted papillomas. In situ hybridization with human papillomavirus RNA probes. Cancer 63, 1708–1713.

    CAS  Google Scholar 

  63. Mounts, P., Shah, K.V., and Kashima, H. (1982) Viral etiology of juvenile- and adult-onset squamous papilloma of the larynx. Proc Natl Acad Sci U S A 79, 5425–5429.

    Article  PubMed  CAS  Google Scholar 

  64. Gabbott, M., Cossart, Y.E., Kan, A., Konopka, M., Chan, R., and Rose, B.R. (1997) Human papillomavirus and host variables as predictors of clinical course in patients with juvenile-onset recurrent respiratory papillomatosis. J Clin Microbiol 35, 3098–3103.

    PubMed  CAS  Google Scholar 

  65. Gissmann, L., Wolnik, L., Ikenberg, H., Koldovsky, U., Schnurch, H.G., and zur Hausen, H. (1983) Human papillomavirus types 6 and 11 DNA sequences in genital and laryngeal papillomas and in some cervical cancers. Proc Natl Acad Sci U S A 80, 560–563.

    Article  PubMed  CAS  Google Scholar 

  66. Syrjanen, K.J. (2003) HPV infections in benign and malignant sinonasal lesions. J Clin Pathol 56, 174–181.

    Article  PubMed  CAS  Google Scholar 

  67. Syrjanen, S., Happonen, R.P., Virolainen, E., Siivonen, L., and Syrjanen, K. (1987) Detection of human papillomavirus (HPV) structural antigens and DNA types in inverted papillomas and squamous cell carcinomas of the nasal cavities and paranasal sinuses. Acta Otolaryngol 104, 334–341.

    Article  PubMed  CAS  Google Scholar 

  68. Hartley, C., Hamilton, J., Birzgalis, A.R., and Farrington, W.T. (1994) Recurrent respiratory papillomatosis--the Manchester experience, 1974-1992. J Laryngol Otol 108, 226–229.

    PubMed  CAS  Google Scholar 

  69. Lie, E.S., Karlsen, F., and Holm, R. (1996) Presence of human papillomavirus in squamous cell laryngeal carcinomas. A study of thirty-nine cases using polymerase chain reaction and in situ hybridization. Acta Otolaryngol 116, 900–905.

    CAS  Google Scholar 

  70. Katori, H., Nozawa, A., and Tsukuda, M. (2005) Markers of malignant transformation of sinonasal inverted papilloma. Eur J Surg Oncol 31, 905–911.

    Article  PubMed  CAS  Google Scholar 

  71. Lele, S.M., Pou, A.M., Ventura, K., Gatalica, Z., and Payne, D. (2002) Molecular events in the progression of recurrent respiratory papillomatosis to carcinoma. Arch Pathol Lab Med 126, 1184–1188.

    PubMed  CAS  Google Scholar 

  72. Hyams, V.J. (1971) Papillomas of the nasal cavity and paranasal sinuses. A clinicopathological study of 315 cases. Ann Otol Rhinol Laryngol 80, 192–206.

    PubMed  CAS  Google Scholar 

  73. Wormald, P.J., Ooi, E., van Hasselt, C.A., and Nair, S. (2003) Endoscopic removal of sinonasal inverted papilloma including endoscopic medial maxillectomy. Laryngoscope 113, 867–873.

    Article  PubMed  Google Scholar 

  74. Lawson, W., Kaufman, M.R., and Biller, H.F. (2003) Treatment outcomes in the management of inverted papilloma: an analysis of 160 cases. Laryngoscope 113, 1548–1556.

    Article  PubMed  Google Scholar 

  75. Califano, J., Koch, W., Sidransky, D., and Westra, W.H. (2000) Inverted sinonasal papilloma : a molecular genetic appraisal of its putative status as a Precursor to squamous cell carcinoma. Am J Pathol 156, 333–337.

    Article  PubMed  CAS  Google Scholar 

  76. Stephen, J.K., Vaught, L.E., Chen, K.M., Sethi, S., Shah, V., Benninger, M.S., et al. (2007) Epigenetic events underlie the pathogenesis of sinonasal papillomas. Mod Pathol 20, 1019–1027.

    Article  PubMed  CAS  Google Scholar 

  77. Yeh, K.T., Chang, J.G., Lin, T.H., Wang, Y.F., Tien, N., Chang, J.Y., et al. (2003) Epigenetic changes of tumor suppressor genes, P15, P16, VHL and P53 in oral cancer. Oncol Rep 10, 659–663.

    PubMed  CAS  Google Scholar 

  78. Shintani, S., Nakahara, Y., Mihara, M., Ueyama, Y., and Matsumura, T. (2001) Inactivation of the p14(ARF), p15(INK4B) and p16(INK4A) genes is a frequent event in human oral squamous cell carcinomas. Oral Oncol 37, 498–504.

    Article  PubMed  CAS  Google Scholar 

  79. Kaghad, M., Bonnet, H., Yang, A., Creancier, L., Biscan, J.C., Valent, A., et al. (1997) Monoallelically expressed gene related to p53 at 1p36, a region frequently deleted in neuroblastoma and other human cancers. Cell 90, 809–819.

    Article  PubMed  CAS  Google Scholar 

  80. Garcia, M., Derocq, D., Freiss, G., and Rochefort, H. (1992) Activation of estrogen receptor transfected into a receptor-negative breast cancer cell line decreases the metastatic and invasive potential of the cells. Proc Natl Acad Sci U S A 89, 11538–11542.

    Article  PubMed  CAS  Google Scholar 

  81. Issa, J.P., Ottaviano, Y.L., Celano, P., Hamilton, S.R., Davidson, N.E., and Baylin, S.B. (1994) Methylation of the oestrogen receptor CpG island links ageing and neoplasia in human colon. Nat Genet 7, 536–540.

    Article  PubMed  CAS  Google Scholar 

  82. Duggan, M.A., Lim, M., Gill, M.J., and Inoue, M. (1990) HPV DNA typing of adult-onset respiratory papillomatosis. Laryngoscope 100, 639–642.

    Article  PubMed  CAS  Google Scholar 

  83. Herman, J.G., Latif, F., Weng, Y., Lerman, M.I., Zbar, B., Liu, S., et al. (1994) Silencing of the VHL tumor-suppressor gene by DNA methylation in renal carcinoma. Proc Natl Acad Sci U S A 91, 9700–9704.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by NIH DE 15990 (MJW).

Author information

Authors and Affiliations

  1. Department of Otolaryngology/Head and Neck Surgery, Henry Ford Hospital, Detroit, MI, USA

    Josena K. Stephen, Kang Mei Chen, Shaleta Havard, Glynis Harris & Maria J. Worsham

Authors
  1. Josena K. Stephen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kang Mei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shaleta Havard
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Glynis Harris
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Maria J. Worsham
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Maria J. Worsham .

Editor information

Editors and Affiliations

  1. School of Medicine, Saba University, Saba, Dutch Antilles

    Ramona G. Dumitrescu

  2. National Institutes of Health (NIH), Div. Cancer Control &, National Cancer Institute, Executive Blvd. 6130Suite 5100, Bethesda, 20892-7324, Maryland, USA

    Mukesh Verma

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Stephen, J.K., Chen, K.M., Havard, S., Harris, G., Worsham, M.J. (2012). Promoter Methylation in Head and Neck Tumorigenesis. In: Dumitrescu, R., Verma, M. (eds) Cancer Epigenetics. Methods in Molecular Biology, vol 863. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-612-8_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-612-8_11

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-611-1

  • Online ISBN: 978-1-61779-612-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation