Abstract
Lipases are widely used in the industry for different purposes. Although these enzymes are mainly produced by submerged fermentation, lipase production by solid-state fermentation (SSF) has been gaining interest due to the advantages of this type of culture. Major advantages are higher production titers and productivity, less catabolite repression, and use of the dried fermented material as biocatalyst. This chapter describes a traditional methodology to produce fungal (Rhizopus homothallicus) lipases by SSF using perlite as inert support. The use of different devices (glass columns or Erlenmeyer flasks) and type of inoculum (spores or growing mycelium) is considered so that lipase production by SSF could be easily performed in any laboratory.
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Acknowledgements
Authors acknowledge CONACyT (México) for the financial support (Project 60005).
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Velasco-Lozano, S., Volke-Sepulveda, T., Favela-Torres, E. (2012). Lipases Production by Solid-State Fermentation: The Case of Rhizopus homothallicus in Perlite. In: Sandoval, G. (eds) Lipases and Phospholipases. Methods in Molecular Biology, vol 861. Humana Press. https://doi.org/10.1007/978-1-61779-600-5_14
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DOI: https://doi.org/10.1007/978-1-61779-600-5_14
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