Abstract
Acetolactate synthase (ALS) is an enzyme in the biosynthetic pathway for branched-chain amino acids, and bispyribac-sodium (BS), a pyrimidinyl carboxy herbicide, is a well-known inhibitor of ALS activity. However, it appears that a mutated form of rice ALS [OsmALS (W548L/S627I)] confers resistance to BS. We succeeded in using OsmALS with native OsALS promoter and terminator region for a selection marker of rice transformation. Because this selection marker cassette is originally from the rice endogenous genome, it can be expected to be publicly acceptable.
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Acknowledgments
We also thank H. Saika, K. Osakabe, K. Abe, S. Nonaka, A. Yokoi, and N. Ohtsuki for discussion, and H. Onodera, K. Ono, K. Amagai, R. Aoto, C. Furusawa, A. Nagashii, E. Ozawa, and F. Suzuki for technical assistance. This work was supported financially by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan to S.T., and a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan to M.E. This work was also supported by a Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN) grant to S.T. and the Budget for Nuclear Research of the Ministry of Education, Culture, Sports, Science and Technology, based on screening and counseling by the Atomic Energy Commission to S.T.
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Endo, M., Shimizu, T., Toki, S. (2012). Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene. In: Dunwell, J., Wetten, A. (eds) Transgenic Plants. Methods in Molecular Biology, vol 847. Humana Press. https://doi.org/10.1007/978-1-61779-558-9_6
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DOI: https://doi.org/10.1007/978-1-61779-558-9_6
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