Abstract
While heavy-ion beam irradiation is becoming popular technology for mutation breeding in Japan, the combination with genetic manipulation makes it more convenient to create greater variation in plant phenotypes. We have succeeded in producing over 200 varieties of transgenic torenia (Torenia fournieri Lind.) from over 2,400 regenerated plants by this procedure in only 2 years. Mutant phenotypes were observed mainly in flowers and showed wide variation in colour and shape. Higher mutation rates in the transgenics compared to those in wild type indicate the synergistic effect of genetic manipulation and heavy-ion beam irradiation, which might be advantageous to create greater variation in floral traits.
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References
Raju, M. R. (1996) Particle radiotherapy: Historical developments and current status, Radiat. Res. 145, 391–407.
Kanaya, T., Saito, H., Hayashi, Y., Fukunishi, N., Ryuto, H., Miyazaki, K., Abe, T., and Suzuki, K. (2008) A heavy-ion beam-induced mutant of Verbena x hybrida and wild species of V. peruviana show different self-incompatibility phenotypes. Plant Biotechnol. 25, 97–100.
Nagatomi, S., Watanabe, H., Tanaka, A., Yamaguchi, H., Degi, K., and Morishita, T. (2003) Six mutant varieties induced by ion beams in chrysanthemum. Instit. Radiat. Breeding, Technical News 65, 1–2.
Imaike, S., Nagayoshi, S., Koriyama, K., and Ueno, K. (2004) Breeding process and characteristics of standard type chrysanthemum cultivar “Araddin”. Bull. Kagoshima Pref. Inst. Agric. Dev., 34, 15–19.
Miyazaki, K., Suzuki, K., Iwaki, K., Kusumi, T., Abe, T., Yoshida, S., and Fukui, H. (2006) Flower pigment mutations induced by heavy ion beam irradiation in an interspecific hybrid of Torenia. Plant Biotechnol. 23, 163–167.
Abe, T., Bae, C.H., Ozaki, T., Wang, K., and Yoshida, S. (2000) Stress-tolerant mutants induced by heavy-ion beams, Gamma Field Symp. 39, 45–54.
Abe, T., Ryuto, H., and Fukunishi, N. (2010) Ion beam radiation mutagenesis, in Plant Mutation Breeding and Biotechnology (Shu, Q.Y., ed.), CABI, Wallingford, Oxfordshire, pp. 95–102.
Aida, R. (2008) Torenia fournieri (torenia) as a model plant for transgenic studies. Plant Biotechnol. 25, 541–545.
Kikuchi, S., Tanaka, H., Shiba, T., Mii, M., and Tsujimoto, H. (2006) Genome size, karyotype, meiosis and a novel extra chromosome in Torenia fournieri, T. baillonii and their hybrid. Chromosome Res. 14, 665–672.
Aida, R. and Shibata, M. (2001) Transgenic Torenia fournieri Lind. (Torenia), in Biotechnology in Agriculture and Forestry, Vol. 48, Transgenic Crops III (Bajaj, Y. P. S., ed.), Springer-Verlag, Berlin, Heidelberg, pp. 294–305.
Aida, R., Kishimoto, S., Tanaka, Y., and Shibata, M. (2000) Modification of flower color in torenia (Torenia fournieri Lind.) by genetic transformation. Plant Sci. 153, 33–42.
Sasaki, K., Aida, R., Niki, T., Yamaguchi, H., Narumi, T., Nishijima, T., Hayashi, Y., Ryuto, H., Fukunishi, N., Abe, T., and Ohtsubo N. (2008) High-efficiency improvement of transgenic torenia flowers by ion beam irradiation. Plant Biotechnol. 25, 81–89.
Sasaki, K., Aida, R., Yamaguchi, H., Shikata, M., Niki, T., Nishijima, T., and Ohtsubo, N. (2010) Functional divergence within class B MADS-box genes TfGLO and TfDEF in Torenia fournieri Lind. Mol. Genet. Genomics 284, 399–414.
Dandekar, A. M. and Fish, H. J. (2005) Plant transformation: Agrobacterium-mediated gene transfer, in Methods in Molecular Biology, vol. 286: Transgenic Plants: Methods and Protocols (Peña, L., ed.) Humana Press Inc., Totowa, NJ, pp. 35–46.
Ryuto, H., Fukunishi, N., Hayashi, Y., Ichida, H., Abe, T., Kase, M., and Yano, Y. (2008) Heavy-ion beam irradiation facility for biological samples in RIKEN. Plant Biotechnol. 25, 199–122.
Kanaya, Y., Miyazaki, K., Suzuki, K., Iwaki, K., Ichida, H., Hayashi, Y., Saito, H., Ryuto, H., Fukunishi, N., and Abe, T. (2006) New cultivar produced by heavy-ion beam irradiation. Proc. of the 25th Symp. on Materials Science and Engineering Res. Cen. of Ion Beam Tech. Hosei Univ., Koganei, Tokyo, pp. 1–6.
Acknowledgments
We thank Dr. Nobuhisa Fukunishi (RIKEN Nishina Center) for the contribution to this work. We also thank Ms. Satoko Ohtawa, Ms. Yoshiko Kashiwagi, and Ms. Yasuko Taniji (National Institute of Floricultural Science) for the skilled technical assistance. This work was supported by the Grant-in-Aid “Research Project for Utilizing Advanced Technologies in Agriculture, Forestry, and Fisheries” from the Research Council, Ministry of Agriculture, Forestry, and Fisheries of Japan (grant no. 1783). This work was also aided by the research projects for the study on genesis of matter from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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Ohtsubo, N. et al. (2012). Efficient Modification of Floral Traits by Heavy-Ion Beam Irradiation on Transgenic Torenia. In: Dunwell, J., Wetten, A. (eds) Transgenic Plants. Methods in Molecular Biology, vol 847. Humana Press. https://doi.org/10.1007/978-1-61779-558-9_24
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DOI: https://doi.org/10.1007/978-1-61779-558-9_24
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