Abstract
Host–pathogen interactions are complex and dynamic processes that result in a variety of responses. The ability of the host to respond appropriately to the presence of a microbial agent defines the outcome of these interactions. Fungal infections are a problem of growing clinical importance and are responsible for serious health problems in multimorbid patients. Different model systems, including primary cells and cell lines derived from different tissues, are used to study several processes that contribute to the virulence of pathogenic fungi. In this chapter, we describe an in vitro assay to characterize the response of human oral keratinocytes (OKF6/TERT-2) to the presence of the human pathogenic fungus, Candida albicans. The dynamic cellular changes such as expression of differentiation markers can be monitored by epifluorescence deconvolution microscopy. Analyses of immunofluorescence data by linescan analysis and fluorescence intensity measurements are described to identify changes in protein expression levels. The use of this in vitro model system will also provide new information about host cell behavior and identify potential drug targets in the future.
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Acknowledgments
We thank Mary C. Young for technical assistance. This work was supported by NSF grant MCB-0517303 (G.M.L.) and NIH grant DE011375 (P.S.). Paula Sundstrom is a Burroughs Wellcome Scholar in Molecular Pathogenic Mycology.
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Wöllert, T., Rollenhagen, C., Langford, G.M., Sundstrom, P. (2012). Human Oral Keratinocytes: A Model System to Analyze Host–Pathogen Interactions. In: Brand, A., MacCallum, D. (eds) Host-Fungus Interactions. Methods in Molecular Biology, vol 845. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-539-8_19
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DOI: https://doi.org/10.1007/978-1-61779-539-8_19
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Publisher Name: Humana, Totowa, NJ
Print ISBN: 978-1-61779-538-1
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