Abstract
The prevalence of fungal infections remains high, and it is associated with significant mortality and morbidity. Macrophages are heterogeneous population of effectors enriched in regions of Candida colonization. These cells sense Candida, and are critical in the resolution of these infections. Here, we describe how macrophages are generated in the presence of colony-stimulating factor-1 (CSF-1); an important cytokine required for the survival, proliferation and ex-vivo differentiation of monocytes to macrophages.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Richardson, M.D. (2005) Changing patterns and trends in systemic fungal infections, J Antimicrob Chemother 56 (Suppl 1), i5–i11.
Lehrnbecher, T., Frank, C., Engels, K.et al. (2010) Trends in the postmortem epidemiology of invasive fungal infections at a university hospital, J Infect 61, 259–65.
Chaffin, W.L., Lopez-Ribot, J.L., Casanova, M. et al. (1998) Cell wall and secreted proteins of Candida albicans: identification, function, and expression, Microbiol Mol Biol Rev 62, 130–80.
Mansour, M.K., Levitz S.M. (2002) Interactions of fungi with phagocytes, Curr Opin Microbiol 5, 359–65.
Villar, C.C., Dongari-Bagtzoglou, A. (2008) Immune defence mechanisms and immunoenhancement strategies in oropharyngeal candidiasis, Expert Rev Mol Med 10, e29.
Newman, S.L., Bhugra, B., Holly, A. et al. (2005) Enhanced killing of Candida albicans by human macrophages adherent to type 1 collagen matrices via induction of phagolysosomal fusion, Infect Immun 73, 770–7.
Baltch, A.L., Bopp, L.H., Smith, R.P. (2005) Effects of voriconazole, granulocyte-macrophage colony-stimulating factor, and interferon gamma on intracellular fluconazole-resistant Candida glabrata and Candida krusei in human monocyte-derived macrophages, Diagn Microbiol Infect Dis 52, 299–304.
Newman, S.L. Holly, A. (2001) Candida albicans is phagocytosed, killed, and processed for antigen presentation by human dendritic cells, Infect Immun 69, 6813–22.
Farah, C.S., Elahi, S., Pang, G. et al. (2001) T cells augment monocyte and neutrophil function in host resistance against oropharyngeal candidiasis, Infect Immun 69, 6110–8.
Torosantucci, A., Romagnoli, G., Chiani, P. et al. (2004) Candida albicans yeast and germ tube forms interfere differently with human monocyte differentiation into dendritic cells: a novel dimorphism-dependent mechanism to escape the host’s immune response, Infect Immun 72, 833–43.
Imamura, K., Dianoux, A., Nakamura, T. et al. (1990) Colony-stimulating factor 1 activates protein kinase C in human monocytes, EMBO J 9, 2423–8, 2389.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Vijayan, D. (2012). Isolation and Differentiation of Monocytes–Macrophages from Human Blood. In: Ashman, R. (eds) Leucocytes. Methods in Molecular Biology, vol 844. Humana Press. https://doi.org/10.1007/978-1-61779-527-5_13
Download citation
DOI: https://doi.org/10.1007/978-1-61779-527-5_13
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-526-8
Online ISBN: 978-1-61779-527-5
eBook Packages: Springer Protocols