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Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue, and Localization of Bacteria with FISH

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Mucins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 842))

Abstract

As mucus is highly hydrated, special care has to be taken to preserve this in histological preparations during immunostaining. Here, we describe how to fix tissues in such a way that the mucus is preserved in paraffin-embedded tissue. We also describe how the major macromolecular components in the mucus, the mucins, are immunostained and how bacteria can be localized in preparations with preserved mucus by fluorescent in situ hybridization.

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References

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Acknowledgments

This work was supported by the Swedish Research Council (No. 7461, 21027, and 342-2004-4434), The Swedish Cancer Foundation, The Knut and Alice Wallenberg Foundation (KAW2007.0118), IngaBritt and Arne Lundberg Foundation, Sahlgren’s University Hospital (LUA-ALF), EU-FP7 IBDase (no. 200931), Wilhelm and Martina Lundgren’s Foundation, Torsten och Ragnar Söderbergs Stiftelser, Swedish CF Foundation, and The Swedish Foundation for Strategic Research—The Mucosal Immunobiology and Vaccine Center (MIVAC) and the Mucus-Bacteria-Colitis Center (MBC) of the Innate Immunity Program (2010–2014).

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Authors and Affiliations

  1. Department of Medical Biochemistry, Mucin Biology Group, University of Gothenburg, Gothenburg, Sweden

    Malin E. V. Johansson & Gunnar C. Hansson

Authors
  1. Malin E. V. Johansson
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  2. Gunnar C. Hansson
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Corresponding author

Correspondence to Gunnar C. Hansson .

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Editors and Affiliations

  1. Mucosal Diseases Program, Mater Medical Research Institute, Aubigny Place, South Brisbane, 4101, Queensland, Australia

    Michael A. McGuckin

  2. Fac. Life Sciences, Wellcome Trust Centre for, University of Manchester, Oxford Road, Manchester, M13 9PT, United Kingdom

    David J. Thornton

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Johansson, M.E.V., Hansson, G.C. (2012). Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue, and Localization of Bacteria with FISH. In: McGuckin, M., Thornton, D. (eds) Mucins. Methods in Molecular Biology, vol 842. Humana Press. https://doi.org/10.1007/978-1-61779-513-8_13

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  • DOI: https://doi.org/10.1007/978-1-61779-513-8_13

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-512-1

  • Online ISBN: 978-1-61779-513-8

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