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Isotope Labeling in Mammalian Cells

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Protein NMR Techniques

Part of the book series: Methods in Molecular Biology ((MIMB,volume 831))

Abstract

Isotope labeling of proteins represents an important and often required tool for the application of nuclear magnetic resonance (NMR) spectroscopy to investigate the structure and dynamics of proteins. Mammalian expression systems have conventionally been considered to be too weak and inefficient for protein expression. However, recent advances have significantly improved the expression levels of these systems. Here, we provide an overview of some of the recent developments in expression strategies for mammalian expression systems in view of NMR investigations.

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Author information

Authors and Affiliations

  1. Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Arpana Dutta & Judith Klein-Seetharaman

  2. Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe-University Frankfurt, Frankfurt am Main, Germany

    Krishna Saxena & Harald Schwalbe

Authors
  1. Arpana Dutta
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  2. Krishna Saxena
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  3. Harald Schwalbe
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  4. Judith Klein-Seetharaman
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Corresponding author

Correspondence to Harald Schwalbe .

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Editors and Affiliations

  1. University of Albany, State University of New York, Washington Avenue 1400, Albany, 12222, New York, USA

    Alexander Shekhtman

  2. University of Albany, State University of New York, Washington Avenue 1400, Albany, 12222, New York, USA

    David S. Burz

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Dutta, A., Saxena, K., Schwalbe, H., Klein-Seetharaman, J. (2012). Isotope Labeling in Mammalian Cells. In: Shekhtman, A., Burz, D. (eds) Protein NMR Techniques. Methods in Molecular Biology, vol 831. Humana Press. https://doi.org/10.1007/978-1-61779-480-3_4

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  • DOI: https://doi.org/10.1007/978-1-61779-480-3_4

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-479-7

  • Online ISBN: 978-1-61779-480-3

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