Abstract
Histone proteins are the major protein components of chromatin – the physiologically relevant form of the genome (or epigenome) in all eukaryotic cells. For many years, histones were considered passive structural components of eukaryotic chromatin. In recent years, it has been demonstrated that dynamic association of histones and their variants to the genome plays a very important role in gene regulation. Histones are extensively modified during posttranslation viz. acetylation, methylation, phosphorylation, ubiquitylation, etc., and the identification of these covalent marks on canonical and variant histones is crucial for the understanding of their biological significance. Different biochemical techniques have been developed to purify and separate histone proteins; here, we describe techniques for analysis of histones from plant tissues.
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References
Higgs, D. R., Vernimmen, D., Hughes, J., Gibbons, R. (2007) Using genomics to study how chromatin influences gene expression. Annual Reviews of Genomics and Human Genetics 8, 299–325.
Ascenzi, R., Gantt, S. (1999) Subnuclear distribution of the entire complement of linker histone variants in Arabidopsis thaliana. Chromosoma 108, 345–355.
Ascenzi, R., Gantt, S. (1999) Molecular genetic analysis of the drought-inducible linker histone variant in Arabidopsis thaliana. Plant Mol Biol 41, 159–169.
Kahn, T. L., Fender, S. E., Bray, E. A., O’ Connell, M. A. (1993) Characterization of expression of drought- and abscisic acid regulated tomato genes in the drought-resistant species Lycopersicon pennellii. Plant Physiology 103, 597–605.
Larochelle, M., Gaudreau, L. (2003) H2A.Z has a function reminiscent of an activator required for preferential binding to intergenic DNA. EMBO J. 22, 4512–4522.
Kouzarides, T. (2007) Chromatin modifications and their functions. Cell 128, 693–705.
Rice, J.C., and Allis, C.D. (2001) Histone methylation versus histone acetylation: new insights into epigenetic regulation. Curr. Opin. Cell Biol. 13, 263–273.
Zhang, Y., and Reinberg, D. (2001) Transcription regulation by histone methylation: interplay between different covalent modifications of the core histone tails. Genes Dev. 15, 2343–2360.
Shi, Y., Lan, F., Matson, C., Mulligan, P., Whetstine, J.R., Cole, P. A., Casero, R. A., Shi, Y. (2004) Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. Cell 119, 941–953.
Tsukada, Y., Fang, J., Erdjument-Bromage, H., Warren, M. E., Borchers, C. H., Tempst, P., Zhang, Y. (2006) Histone demethylation by a family of JmjC domain-containing proteins, Nature 439, 811–816.
Jiang, D., Yang, W., He, Y., Amasino, R. M. (2007) Arabidopsis relatives of the human lysine-specific demethylase1 repress the expression of FWA and FLOWERING LOCUS C and thus promote the floral transition. The Plant Cell 19, 2975–2987.
Wade, P. A. (2001) Transcriptional control at regulatory checkpoints by histone deacetylases: molecular connections between cancer and chromatin. Hum. Mol.Genet. 10, 693–698.
Pandey, R., Muller, A., Napoli, C.A., Selinger, D. A., Pikaard, C. S., Richards, E. J., Bender, J., Mount, D. W., Jorgensen, R. A. (2002) Analysis of histone acetyltransferase and histone deacetylase families of Arabidopsis thaliana suggests functional diversification of chromatin modification among multicellular eukaryotes. Nucleic Acids Research 30, 5036–5055.
Ruijter, A. J. M., Gennip, A. H. V., Caron, H. N., Kemp S., Kuilenburg, A. B. P. V. (2003) Histone deacetylases (HDACs): characterisation of the classical HDAC family. Biochem. J. 370, 737–749.
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The work has been supported by Council of Scientific and Industrial Research, India, under the project OLP0031.
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Trivedi, I. et al. (2012). Analysis of Histones and Histone Variants in Plants. In: Morse, R. (eds) Chromatin Remodeling. Methods in Molecular Biology, vol 833. Humana Press. https://doi.org/10.1007/978-1-61779-477-3_14
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DOI: https://doi.org/10.1007/978-1-61779-477-3_14
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