Skip to main content
SpringerLink
Log in

ADHD Animal Model Characterization: Transcriptomics and Proteomics Analyses

  • Protocol
  • First Online:
Psychiatric Disorders

Part of the book series: Methods in Molecular Biology ((MIMB,volume 829))

Abstract

Mechanisms underlying behavioral abnormalities of patients with attention-deficit hyperactivity disorder (ADHD) disorder are still unknown. It is worth clarifying alterations in the brain of animal models for ADHD. The animals with neonatal treatment with 6-hydroxydopamine (6-OHDA) and congenic wiggling (Wig) rats show motor hyperactivities during a period of darkness at 4 weeks of age. In rats with 6-OHDA lesions, subcutaneous injection of methamphetamine attenuates hyperactivity, the reverse of its effect in Wig rats. To understand mechanisms underlying such behavioral abnormalities, transcriptomics and proteomics analyses may provide novel information in brain research. The expression of genes and proteins in brain regions can be measured by DNA microarray and two-dimensional gel electrophoresis, respectively. We have shown different expressions of genes and proteins in brains of rats with neonatal 6-OHDA lesions and Wig rats.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Viggiano, D., Vallone, D., Ruocco, L. A. and Sadile, A. G. (2003) Behavioural, pharmacological, morpho-functional molecular studies reveal a hyperfunctioning mesocortical dopamine system in an animal model of attention deficit and hyperactivity disorder. Neurosci. Biobehav. Rev. 27, 683–689.

    Article  PubMed  CAS  Google Scholar 

  2. Shaywitz, B. A., Yager, R. D. and Klopper, J. H. (1976) Selective brain dopamine depletion in developing rats: an experimental model of minimal brain dysfunction. Science 191, 305–308.

    Article  PubMed  CAS  Google Scholar 

  3. Shaywitz, B. A., Klopper, J. H., Yager, R. D. and Gordon J. W. (1976) Paradoxical response to amphetamine in developing rats treated with 6- hydroxydopamine. Nature 261, 153–155.

    Article  PubMed  CAS  Google Scholar 

  4. Swanson, J. M., Sergeant, J. A., Taylor, E., Sonuga-Barke, E. J., Jensen, P. S. and Cantwell, D. P. (1998) Attention-deficit hyperactivity disorder and hyperkinetic disorder. Lancet 351, 429–433.

    Article  PubMed  CAS  Google Scholar 

  5. Volkow, N. D., Fowler, J. S., Wang, G. J., Ding, Y. S. and Gatley, S. J. (2002) Role of dopamine in the therapeutic and reinforcing effects of methylphenidate in humans: results from imaging studies. Eur. Neuropsychopharmacol. 12, 557–566.

    Article  PubMed  CAS  Google Scholar 

  6. Kamimura, E., Ueno, Y., Tanaka, S., Sawa, H., Yoshioka, M., Ueno, K. I., Inoue, T., Li, X., Koyama, T., Ishikawa, R. and Nagashima, K. (2001) New rat model for attention deficit hyperactive disorder (ADHD). Comp. Med. 51, 245–251.

    PubMed  CAS  Google Scholar 

  7. Masuo, Y., Ishido, M., Morita, M., Oka, S. and Niki, E. (2004) Motor activity and gene expression in rats with neonatal 6-hydroxydopamine lesions. J. Neurochem. 91, 9–19.

    Article  PubMed  CAS  Google Scholar 

  8. Masuo, Y., Ishido, M., Morita, M., Sawa, H., Nagashima, K., Oka, S. and Niki, E. (2007) Behavioural characteristics and gene expression in the hyperactive wiggling (Wig) rat. Eur. J. Neurosci. 25, 3659–3666.

    Article  PubMed  Google Scholar 

  9. Hirano, M., Rakwal, R., Shibato, J., Sawa, H., Nagashima, K., Ogawa, Y., Yoshida, Y., Iwahashi, H., Niki, E. and Masuo, Y. (2008) Proteomics- and transcriptomics-based screening of differentially expressed proteins and genes in brain of Wig rat: A model for attention deficit hyperactivity disorder (ADHD) research. J. Proteome Res. 7, 2471–2499.

    Article  PubMed  CAS  Google Scholar 

  10. Hirano, M., Rakwal, R., Shibato, J., Agrawal, G. K., Jwa, N. S., Iwahashi, H. and Masuo, Y. (2006) New protein extraction/solubilization protocol for gel-based proteomics of rat (female) whole brain and brain regions. Mol. Cells 22, 119–125.

    PubMed  CAS  Google Scholar 

  11. Hirano, M., Rakwal, R., Kouyama, N., Katayama, Y., Hayashi, M., Shibato, J., Ogawa, Y., Yoshida, Y., Iwahashi, H. and Masuo, Y. (2007) Gel-based proteomics of unilateral irradiated striatum after gamma knife surgery. J. Proteome Res. 6, 2656–2668.

    Article  PubMed  CAS  Google Scholar 

  12. Masuo, Y., Matsumoto, Y., Morita, S. and Noguchi, J. (1997) A novel method for counting spontaneous motor activity in the rat. Brain Res. Protocols 1, 321–326.

    Article  CAS  Google Scholar 

  13. Rosenzweig, B. A., Pine, P. S., Domon, O. E., Morris, S. M., Chen, J. J. and Sistare, F. D. (2004) Dye bias correction in dual-labeled cDNA microarray gene expression measurements. Environ. Health Perspect. 112, 480–487.

    Article  PubMed  CAS  Google Scholar 

  14. Altman, N. (2005) Replication, variation and normalisation in microarray experiments. Appl. Bioinformatics 4, 33–44.

    Article  PubMed  CAS  Google Scholar 

  15. Martin-Magniette, M. L., Aubert, J., Cabannes, E. and Daudin, J. J. (2005) Evaluation of the gene-specific dye bias in cDNA microarray experiments. Bioinformatics 21, 1995–2000.

    Article  PubMed  CAS  Google Scholar 

  16. Cho, K., Agrawal, G. K., Shibato, J., Jung, Y. H., Kim, Y. K., Nahm, B. H., Jwa, N. S., Tamogami, S., Han, O., Kohda, K., Iwahashi, H. and Rakwal, R. (2007) Survey of differentially expressed proteins and genes in jasmonic acid treated rice seedling shoot and root at the proteomics and transcriptomics levels. J. Proteome Res. 6, 3581–3603.

    Article  PubMed  CAS  Google Scholar 

  17. Cho, K., Shibato, J., Agrawal, G. K., Jung, Y. H., Kubo, A., Jwa, N. S., Tamogami, S., Satoh, K., Kikuchi, S., Higashi, T., Kimura, S., Saji, H., Tanaka, Y., Iwahashi, H., Masuo, Y. and Rakwal, R. (2008) Integrated transcriptomics, proteomics, and metabolomics analyses to survey ozone responses in the leaves of rice seedling. J. Proteome Res. 7, 2990–2998.

    Article  Google Scholar 

  18. Perkins, D. N., Pappin, D. J., Creasy, D. M. and Cottrell, J. S. (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20, 3551–3567.

    Article  PubMed  CAS  Google Scholar 

  19. Shevchenko, A., Wilm, M., Vorm, O. and Mann, M. (1996) Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem. 68, 850–858.

    Article  PubMed  CAS  Google Scholar 

  20. Jung, Y. H., Rakwal, R., Agrawal, G. K., Shibato, J., Kim, J. A., Lee, M. O., Choi, P. K., Jung, S. H., Kim, S. H., Koh, H. J., Yonekura, M., Iwahashi, H. and Jwa, N. S. (2006) Differential expression of defense/stress-related marker proteins in leaves of a unique rice blast lesion mimic mutant (blm). J. Proteome Res. 5, 2586–2598.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported in part by a fellowship to Y.M. from the New Energy and Industrial Technology Development Organization (NEDO) and by the Ministry of the Environment; and the Ministry of Economy, Trade, and Industry, Japan. We appreciate the support of Drs. Hyung Wook Nam (Protein Network Research Center, Yonsei University, Seoul, Korea), Yoko Ogawa and Yasukazu Yoshida (Health Technology Research Center, AIST, Ikeda, Osaka, Japan) in use of the MALDI-MS and LC-MS/MS equipments. RR also appreciates the kind support of Prof. Seiji Shioda (Department of Anatomy I, Showa University-School of Medicine) for his encouragement on multidisciplinary research and study.

Author information

Authors and Affiliations

  1. Laboratory of Neuroscience, Department of Biology, Toho University, Funabashi, Chiba, Japan

    Yoshinori Masuo & Junko Shibato

  2. School of Medicine, Showa University, Shinagawa, Tokyo, Japan

    Randeep Rakwal

Authors
  1. Yoshinori Masuo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Junko Shibato
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Randeep Rakwal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yoshinori Masuo .

Editor information

Editors and Affiliations

  1. McKnight Brain Institute, Department of Psychiatry, University of Florida, S. Newell Dr. 100, Gainesville, 32610, Florida, USA

    Firas H. Kobeissy

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Masuo, Y., Shibato, J., Rakwal, R. (2012). ADHD Animal Model Characterization: Transcriptomics and Proteomics Analyses. In: Kobeissy, F. (eds) Psychiatric Disorders. Methods in Molecular Biology, vol 829. Humana Press. https://doi.org/10.1007/978-1-61779-458-2_32

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-458-2_32

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-457-5

  • Online ISBN: 978-1-61779-458-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation