Abstract
Microglia are the principal immune effector cells of the central nervous system (CNS). Under normal conditions, they occupy a quiescent surveillance phenotype, but following stimulation by microorganisms or inflammatory cytokines, microglia transform into highly activated migratory, phagocytic cells producing inflammatory cytokines and chemokines. Significantly, several studies have demonstrated that astrocytes attenuate microglial activation, reducing microglial adhesion, production of interleukin-12 (IL-12) and reactive oxygen species (ROS), and expression of inducible nitric oxide synthase (iNOS). In this chapter, we describe an astrocyte-microglia coculture system that can be used to investigate interactions between these two cell types. We also describe a flow cytometry approach to quantify microglial activation state, as assessed by microglial expression of cellular activation markers, including MHC class I and the Mac-1 and α4 integrins
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Acknowledgments
This work was supported by the National Multiple Sclerosis Society; Harry Weaver Neuroscience Scholar Award (RM), and Postdoctoral Fellowship (JVW), and by the NIH grant RO1 NS060770.
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Welser, J.V., Milner, R. (2012). Use of Astrocyte-Microglial Cocultures to Examine the Regulatory Influence of Astrocytes on Microglial Activation. In: Milner, R. (eds) Astrocytes. Methods in Molecular Biology, vol 814. Humana Press. https://doi.org/10.1007/978-1-61779-452-0_24
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DOI: https://doi.org/10.1007/978-1-61779-452-0_24
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