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Isotope-Based Quantitation of Uptake, Release, and Metabolism of Glutamate and Glucose in Cultured Astrocytes

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Astrocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 814))

Abstract

Protocols are described for measurement in primary cultures of astrocytes of unidirectional fluxes of glutamate (influx and efflux), glutamate metabolism to glutamine or CO2, glucose influx, glycolysis, pyruvate dehydrogenation, oxidative metabolism of glucose, pyruvate carboxylation, glycogen synthesis, and glycogenolysis. References are made to the in vivo situation, and the importance of using metabolically competent cultures is emphasized.

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Authors and Affiliations

  1. College of Basic Medical Sciences, China Medical University, Shenyang, People’s Republic of China

    Leif Hertz

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  1. Leif Hertz
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Correspondence to Leif Hertz .

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Editors and Affiliations

  1. Dept. Molecular & Experimental, Scripps Research Institute, N. Torrey Pines Road 10550, La Jolla, 92037, California, USA

    Richard Milner

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Hertz, L. (2012). Isotope-Based Quantitation of Uptake, Release, and Metabolism of Glutamate and Glucose in Cultured Astrocytes. In: Milner, R. (eds) Astrocytes. Methods in Molecular Biology, vol 814. Humana Press. https://doi.org/10.1007/978-1-61779-452-0_20

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  • DOI: https://doi.org/10.1007/978-1-61779-452-0_20

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-451-3

  • Online ISBN: 978-1-61779-452-0

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