Abstract
Genetic lineage tracing (or fate mapping) techniques are designed to permanently label progenitor cells of target tissues, thereby allowing delineation of the progenies of labeled cells during organogenesis. This technology has been widely used in the study of cell migration and lineage specification in various organs and organisms. Here, we describe how to apply the genetic lineage tracing model in combination with immunohistochemistry to identify the potential origins of somatic cell precursors in perinatal mouse ovaries.
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Acknowledgments
This research was supported by the National Institute of Health (HD046861, HD059661, and ES018163). It was also supported in part by the Intramural Research Program of the National Institute of Environmental Health Sciences (NIEHS) and NIH Graduate Partnership Program.
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Liu, C., Paczkowski, M., Othman, M., Yao, H.HC. (2012). Investigating the Origins of Somatic Cell Populations in the Perinatal Mouse Ovaries Using Genetic Lineage Tracing and Immunohistochemistry. In: Chan, WY., Blomberg, L. (eds) Germline Development. Methods in Molecular Biology, vol 825. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-436-0_16
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DOI: https://doi.org/10.1007/978-1-61779-436-0_16
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