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Ovariectomy/Orchidectomy in Rodents

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Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 816))

Abstract

This chapter describes the surgical procedures for ovariectomy and orchidectomy in mice and rats. In ­addition to providing technical details of the surgical techniques, details of anaesthesia and perioperative care are also included.

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References

  1. Khosla, S., Melton, L. J. III, and Riggs, B. L. (2001) Estrogens and bone health in men. Calcif. Tissue Int. 69, 189–192.

    Article  PubMed  CAS  Google Scholar 

  2. Khosla, S., Melton, L. J. III, Atkinson, E. J., O’Fallon, W. M., Klee, G. G., and Riggs, B. L. (1998) Relationship of serum sex steroid levels and bone turnover markers with bone mineral density in men and women: a key role for bioavailable estrogen. J. Clin. Endocrinol. Metab. 83, 2266–2274.

    Article  PubMed  CAS  Google Scholar 

  3. Samuels, A., Perry, M. J., and Tobias, J. H. (1999) High-dose estrogen induces de novo medullary bone formation in female mice. J. Bone Miner. Res. 14, 178–186.

    Article  PubMed  CAS  Google Scholar 

  4. Tobias, J. H., and Compston, J. E. (1999) Does estrogen stimulate osteoblast function in postmenopausal women? Bone 24, 121–124.

    Article  PubMed  CAS  Google Scholar 

  5. Weinstein, R. S., and Manolagas, S. C. (2000) Apoptosis and osteoporosis. Am. J. Med. 108, 153–164.

    Article  PubMed  CAS  Google Scholar 

  6. Raisz, L. G. (2005) Pathogenesis of osteoporosis: concepts, conflicts, and prospects. J. Clin. Invest. 115, 3318–3325.

    Article  PubMed  CAS  Google Scholar 

  7. Idris, A. I., Van’t Hof, R. J., Greig, I. R., Ridge, S. A., Baker, D., Ross, R. A., and Ralston, S. H. (2005) Regulation of bone mass, bone loss and osteoclast activity by cannabinoid receptors. Nat. Med. 11, 774–779.

    Article  PubMed  CAS  Google Scholar 

  8. Rissanen, J. P., Suominen, M. I., Peng, Z., Morko, J., Rasi, S., Risteli, J., and Halleen, J. M. (2008) Short-term changes in serum PINP predict long-term changes in trabecular bone in the rat ovariectomy model. Calcif. Tissue Int. 82, 155–161.

    Article  PubMed  CAS  Google Scholar 

  9. Khosla, S., Melton, L. J. III, and Riggs, B. L. (2001) Estrogens and bone health in men. Calcif. Tissue Int. 69, 189–192.

    Article  PubMed  CAS  Google Scholar 

  10. Gunness, M., and Orwoll, E. (1995) Early induction of alterations in cancellous and cortical bone histology after orchiectomy in mature rats. J. Bone Miner. Res. 10, 1735–1744.

    Article  PubMed  CAS  Google Scholar 

  11. Danielsen, C. C., Mosekilde, L., and Andreassen, T. T. (1992) Long-term effect of orchidectomy on cortical bone from rat femur: bone mass and mechanical properties. Calcif. Tissue Int. 50, 169–174.

    Article  PubMed  CAS  Google Scholar 

  12. Orwoll, E. S. (1996) Androgens as anabolic agents for bone. Trends Endocrinol. Metab. 7, 77–84.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgement

The author would like to thank Dr. Antonia Sophocleous PhD for providing the images for Figs. 1 and 2 and for critically reviewing the manuscript.

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Correspondence to Aymen I. Idris .

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© 2012 Springer Science+Business Media, LLC

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Idris, A.I. (2012). Ovariectomy/Orchidectomy in Rodents. In: Helfrich, M., Ralston, S. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 816. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-415-5_34

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  • DOI: https://doi.org/10.1007/978-1-61779-415-5_34

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-414-8

  • Online ISBN: 978-1-61779-415-5

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