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Design of Biomolecules for Nanoengineered Biomaterials for Regenerative Medicine

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Nanotechnology in Regenerative Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 811))

Abstract

An important goal in the development of highly functional organic materials is to design self-assembling molecules that can reproducibly display chemical signals across length scales. Within the biomedical field, biomolecules are highly attractive candidates to serve as bioactive building blocks for the next generation of biomaterials. The peptide amphiphiles (PAs) developed by the Stupp Laboratory at Northwestern University generated a highly versatile self-assembly code to create well-defined bioactive nanofibers that have been proven to be very effective at signaling cells in vitro and in vivo. Here, we describe the basic steps necessary for synthesis and assembly of PA molecules into functional nanostructures.

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References

  1. Huebsch, N., Mooney, D. (2009) Inspiration and application in the evolution of biomaterials Nature 462, 426–432.

    CAS  Google Scholar 

  2. Stevens, M.M., George, J.H. (2005) Exploring and engineering the cell surface interface Science 310, 1135–1138.

    CAS  Google Scholar 

  3. Furth, M. E., Atala, A. (2008) Current and future perspectives of regenerative medicine. Principles of Regenerative Medicine. Burlington MA: Elsevier.

    Google Scholar 

  4. Stupp, S. I. (2005) Biomaterials for regenerative medicine MRS Bulletin 30, 546–553.

    Article  CAS  Google Scholar 

  5. Hartgerink, J.D., Beniash, E., Stupp, S. I. (2001) Self-assembly and mineralization of peptide-amphiphile nanofibers Science 294, 1684–1688.

    CAS  Google Scholar 

  6. Hartgerink, J.D., Beniash, E., Stupp, S.I. (2002) Peptide-amphiphile nanofibers: A versatile scaffold for the preparation of self-assembling materials Proceedings of the National Academy of Sciences of the United States of America 99, 5133–5138.

    CAS  Google Scholar 

  7. Paramanov, S.E., Gauba, V., Hartgerink, J.D. (2005) Synthesis of collagen-like peptide polymers by native chemical ligation Macromolecules 38, 7555–7561.

    Google Scholar 

  8. Betre, H., Setton, L.A., Meyer, D.E., Chilkoti, A. (2002) Characterization of a genetically engineered elastin-like polypeptide for cartilaginous tissue repair Biomacromolecules 3, 910–916.

    CAS  Google Scholar 

  9. Zhao, X., Zhang, S. (2007) Designer self-assembling peptide materials Macromolecular Biosciences 7, 13–22.

    Google Scholar 

  10. Aggeli, A., Bell, M., Carrick, L.M., Fishwick, C.W.G., Harding, R., Mawer, P.J., Radford, S.E., Strong, A.E., Boden, N. (2003) pH as a trigger of peptide b-sheet self-assembly and reversible switching between nematic and isotropic phases Journal of the American Chemical Society 125, 9619–9628.

    Article  CAS  Google Scholar 

  11. Williams, R.J., Smith, A.M., Collins, R., Hodson, N., Das, A.K., Ulijn, R.V. (2009) Enzyme-assisted self-assembly under thermodynamic control Nature Nanotechnology 4, 19–24.

    CAS  Google Scholar 

  12. Schneider, J.P., Pochan, D.J., Ozbas, B., Rajagopal, K., Pakstis, L., Kretsinger, J. (2002) Responsive hydrogels from the intramolecular folding and self-assembly of a designed peptide Journal of the American Chemical Society 124, 15030–15037.

    CAS  Google Scholar 

  13. Zhang, S., Holmes, T., Lockshin, C., Rich, A. (1993) Spontaneous assembly of a self-complementary oligopeptide to form a stable macroscopic membrane Proceeding of the National Academy of Sciences of the United States of America 90, 3334–3338.

    Article  CAS  Google Scholar 

  14. Silva, G.A., Czeisler, C., Niece, K.L., Beniash, E., Harrington, D.A., Kessler, J. A., Stupp, S. I. (2004) Selective differentiation of neural progenitor cells by high-epitope density nanofibers Science 303(5662), 1352–1355.

    CAS  Google Scholar 

  15. Guler, M.O., Soukasene, S., Hulvat, J.F., Stupp, S.I. (2005) Presentation and recognition of biotin on nanofibers formed by branched peptide amphiphiles. Nano Letters 5(2), 249–252.

    Article  CAS  Google Scholar 

  16. Rajangam, K., Behanna, H.A., Hui, M.J., Han, X., Hulvat, J.F., Lomasney, J.W., Stupp, S.I. (2006) Heparin binding nanostructures to promote growth of blood vessels Nano Letters 6, 2086–2090.

    CAS  Google Scholar 

  17. Storrie, H., Guler, M.O., Abu-Amara, S.N., Volberg, T., Rao, M., Geiger, B., Stupp, S.I. (2007) Supramolecular crafting of cell adhesion Biomaterials 28, 4608–4618.

    CAS  Google Scholar 

  18. Stendahl, J.C., Want, L.J., Chow, L.W., Kaufman, D.B., Stupp, S.I. (2008) Growth factor delivery from self-assembling nanofibers to facilitate islet transplantation Transplantation 86(3), 478–481.

    Article  CAS  Google Scholar 

  19. Capito, R., Azevedo, H., Gelichko, Y., Mata, A., Stupp, S.I. (2008) Self-assembly of large and small molecules into hierarchically ordered sacs and membranes Science 319, 1812–1816.

    CAS  Google Scholar 

  20. Kapadia, M. R., Chow, L.W., Tsihlis, N.D., Ahanchi, S.S., Eng, J.W., Murar, J., Martinez, J., Popowich, D.A., Jiang, Q., Hrabie, J.A., Saavedra, J.E., Keefer, L.K., Hulvat, J.F., Stupp, S.I., Kibbe, M.R. (2008) Nitric oxide and nanotechnology: A novel approach to inhibit neointimal hyperplasia Journal of Vascular Surgery 47(1), 173–182.

    Google Scholar 

  21. Tysseling-Mattiace, V.M., Sahni, V., Niece, K.L., Birch, D., Czeisler, C., Fehlings, M.G., Stupp, S.I., Kessler, J.A. (2008) Self-assembling nanofibers inhibit glial scar formation and promote axon elongation after spinal cord injury The Journal of Neuroscience 28(14), 3814–3823.

    CAS  Google Scholar 

  22. Rajangam, K., Arnold, M.S., Rocco, M.A., Stupp, S.I. (2008) Peptide amphiphile nanostructure-heparin interactions and their relationship to bioactivity Biomaterials 29, 293298–293305.

    Google Scholar 

  23. Sargeant, T.D., Guler, M.O., Oppenheimer, S.M., Mata, A., Satcher, R.L., Dunand, D.C., Stupp, S.I. (2008) Hybrid bone implants: Self-assembly of peptide amphiphile nanofibers within porous titanium Biomaterials 29(2), 161–171.

    CAS  Google Scholar 

  24. Sargeant, T. D., Rao, M.S., Koh, C.Y., Stupp, S.I. (2008) Covalent functionalization of NiTi surfaces with bioactive peptide amphiphile nanofibers Biomaterials 29(8), 1085–1098.

    CAS  Google Scholar 

  25. Spoerke, E.D., Anthony, S.G., Stupp, S.I. (2009) Enzyme Directed Templating of Artificial Bone Mineral Advanced Materials 21(4), 425–430.

    CAS  Google Scholar 

  26. Mata, A., Hsu, L., Capito, R., Aparicio, C., Henrikson, K., Stupp, S.I. (2009) Micropatterning of bioactive self-assembling gels Soft Matter 5, 1228–1236.

    Article  CAS  Google Scholar 

  27. Webber, M.J., Tongers, J., Renault, M.A., Roncalli, J.G., Losordo, D.W., Stupp, S.I. (2009) Development of bioactive peptide amphiphiles for therapeutic cell delivery Acta Biomateriala 6, 3–11.

    Google Scholar 

  28. Mata, A., Geng, Y., Henrikson, K., Aparicio, C., Stock, S., Satcher, R., Stupp, S.I. (2010) Bone regeneration mediated by biomimetic mineralization of a nanofiber matrix Biomaterials 31, 6004–6012.

    CAS  Google Scholar 

  29. Shah, R.N., Shah, N.A., Del Rosario Lim, M.M., Hsieh, C., Nuber, G., Stupp, S.I. (2010) Supramolecular design of self-assembling nanofibers for cartilage regeneration Proceedings of the National Academy of Sciences of the United States of America 107(8), 3293–3298.

    CAS  Google Scholar 

  30. Chow, L.W., Wang, L.J., Kaufman, D.B., Stupp, S.I. (2010) Self-assembling nanostructures to deliver angiogenic factors to pancreatic islets Biomaterials 31(24), 6154–6161.

    CAS  Google Scholar 

  31. Standley, S.M., Toft, D.T., Cheng, H., Soukasene, S., Chen, J., Raja, S.M., Band, V., Band, H., Cryns, V.L, Stupp, S.I. Induction of Cancer Cell Death by Self-assembling Nanostructures Incorporating a Cytotoxic Peptide Cancer Research. (available online Res. 0: 0008–5472.CAN-09-3267v1).

    Google Scholar 

  32. Muraoka, T., Koh, C.Y., Cui, H., Stupp, S.I. (2009) Light-triggered bioactivity in three-dimensions Angewante Chemie International Edition 48, 5946–5949.

    CAS  Google Scholar 

  33. Ghanaati, S., Webber, M.J., Unger, R.E., Orth, C., Hulvat, J.F., Kiehna, S.E., Barbeck, M., Rasic, A., Stupp, S.I. (2009) Dynamic in vivo biocompatibility of angiogenic peptide amphiphile nanofibers Biomaterials 30, 6202–6212.

    CAS  Google Scholar 

  34. Behanna, H.A., Donners, J.J.J.M, Gordon, A.C., Stupp, S.I. (2005) Coassembly of amphiphiles with opposite polarities into nanofibers Journal of the American Chemical Society 127, 1193–1200.

    Article  CAS  Google Scholar 

  35. Greenfield, M.A., Hoffman, J.R., de la Cruz, M.O., Stupp, S.I. (2010) Tunable Mechanics of Peptide Nanofiber Gels Langmuir 26(5), 3641–3647.

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. The Nanotechnology Platform, Parc Científic Barcelona, Barcelona, Spain

    Alvaro Mata & Esther Tejeda-Montes

  2. Department of Chemistry, Northwestern University, Evanston, IL, USA

    Liam Palmer

  3. Department of Chemistry, Northwestern University, Evanston, IL, USA

    Samuel I. Stupp

  4. Department of Materials Science and Engineering, Northwestern University, Evanston, IL, USA

    Samuel I. Stupp

  5. Institute for BioNanotechnology in Medicine, Northwestern University, Evanston, IL, USA

    Samuel I. Stupp

Authors
  1. Alvaro Mata
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  2. Liam Palmer
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  3. Esther Tejeda-Montes
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  4. Samuel I. Stupp
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Corresponding author

Correspondence to Alvaro Mata .

Editor information

Editors and Affiliations

  1. (IBEC), Inst. for Bioengineering of Catalonia, C/Baldiri Reixac 10-12, Barcelona, 08028, Spain

    Melba Navarro

  2. (IBEC), Inst. for Bioengineering of Catalonia, C/Baldiri Reixac 10-12, Barcelona, 08028, Spain

    Josep A. Planell

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© 2012 Springer Science+Business Media, LLC

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Mata, A., Palmer, L., Tejeda-Montes, E., Stupp, S.I. (2012). Design of Biomolecules for Nanoengineered Biomaterials for Regenerative Medicine. In: Navarro, M., Planell, J. (eds) Nanotechnology in Regenerative Medicine. Methods in Molecular Biology, vol 811. Humana Press. https://doi.org/10.1007/978-1-61779-388-2_3

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  • DOI: https://doi.org/10.1007/978-1-61779-388-2_3

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-387-5

  • Online ISBN: 978-1-61779-388-2

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