Abstract
Alzheimer’s disease, the most common neurodegenerative disease, is characterized by a progressive loss of synapses and accumulation of amyloid-beta (Aβ) peptides in the brain. Previous studies demonstrated that acute increase in synaptic activity in cultured hippocampal slices and mouse brains (Cirrito et al. Neuron 48: 913–922, 2005; Kamenetz et al. Neuron 37: 925–937, 2003) enhanced secretion of Aβ. Since synaptic activity promotes Aβ secretion, it could also affect the trafficking and processing of its precursor, the amyloid precursor protein (APP). Here, we describe a method to investigate the effect of acute synaptic activation on APP trafficking within dendrites.
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Acknowledgments
Supported by an Alzheimer’s Association New Investigator Award (DT) and Zenith Award (GKG) and National Institute of Health grants AG027140 and AG028174 (GKG). We thank Dr. Carlos Dotti, Catholic University of Leuven, Belgium, for providing the APP–YFP construct.
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Tampellini, D., Gouras, G.K. (2011). Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging. In: Manfredi, G., Kawamata, H. (eds) Neurodegeneration. Methods in Molecular Biology, vol 793. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-328-8_22
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DOI: https://doi.org/10.1007/978-1-61779-328-8_22
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