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Native Chromatin Immunoprecipitation

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Epigenetics Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 791))

Abstract

Native chromatin immunoprecipitation refers to a method that allows for identification and quantification of DNA that is associated with specific chromatin proteins without altering the structure of these proteins. The method has been used with great success in the past and has some advantages over the more widely used cross-linking chromatin immunoprecipitation. We describe here a protocol that was specifically optimized for low cell numbers.

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Correspondence to Christoph Grunau .

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Cosseau, C., Grunau, C. (2011). Native Chromatin Immunoprecipitation. In: Tollefsbol, T. (eds) Epigenetics Protocols. Methods in Molecular Biology, vol 791. Humana Press. https://doi.org/10.1007/978-1-61779-316-5_15

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  • DOI: https://doi.org/10.1007/978-1-61779-316-5_15

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-315-8

  • Online ISBN: 978-1-61779-316-5

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