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Phosphoproteomics Using iTRAQ

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Plant Kinases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 779))

Abstract

The identification of phosphorylation on proteins has become practicable for many laboratories in recent years, largely due to improvements in mass spectrometry (MS) and the development of methods to selectively enrich for phosphorylated peptides and proteins. However, phosphorylation is a dynamic and reversible modification which plays a central role in many biological processes including intracellular signalling. Therefore, the quantitative analysis of phosphorylated proteins and peptides is a subject of intense interest. We discuss three applications of isobaric tags for relative and absolute quantitation (iTRAQ) to the analysis of phosphopeptides from a variety of sample materials.

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Acknowledgements

A.M.E.J. would like to thank Yong-Xi Chen (Jiaotong University, Shanghai) and Rod Watson (Applied Biosystems) for useful discussions. A.M.E.J. is funded by the Gatsby Charitable Foundation and T.S.N. by a BBSRC David Phillips Fellowship.

Author information

Authors and Affiliations

  1. The Sainsbury Laboratory, Norwich Research Park, Norwich, UK

    Alexandra M. E. Jones

  2. Faculty of Life Sciences, University of Manchester, Manchester, UK

    Thomas S. Nühse

Authors
  1. Alexandra M. E. Jones
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  2. Thomas S. Nühse
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Corresponding author

Correspondence to Alexandra M. E. Jones .

Editor information

Editors and Affiliations

  1. Leibniz Institute of Plant Biochemistry, Weinberg 3, Halle (Saale), 06120, Sachsen-Anhalt, Germany

    Nico Dissmeyer

  2. rue du Général Zimmer 12, Strasbourg, F-67000, France

    Arp Schnittger

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Jones, A.M.E., Nühse, T.S. (2011). Phosphoproteomics Using iTRAQ. In: Dissmeyer, N., Schnittger, A. (eds) Plant Kinases. Methods in Molecular Biology, vol 779. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-61779-264-9_17

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  • DOI: https://doi.org/10.1007/978-1-61779-264-9_17

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  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-61779-263-2

  • Online ISBN: 978-1-61779-264-9

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