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Profiling of Hormones and Related Metabolites in Seed Dormancy and Germination Studies

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Seed Dormancy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 773))

Abstract

Seed dormancy and germination are regulated by several plant hormones, such as abscisic acid, gibberellin, auxin (indole-3-acetic acid), ethylene, and brassinosteroid. Endogenous concentrations of a hormone are determined by the balance between biosynthesis and deactivation, and contribute to the regulation of physiological responses. Therefore, profiling of all hormones and their metabolites (hormonome) is a powerful approach to elucidate the regulatory networks of hormone metabolism. The methods involved in the use of liquid chromatography–electrospray ionization–tandem mass spectrometry to develop a high-sensitive and high-throughput hormonome platform are described in this chapter.

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Acknowledgments

The hormone analysis platform described in this chapter has been developed by members in Growth Regulation Research Group, RIKEN Plant Science Center: Yuji Kamiya, Shinjiro Yamaguchi, Eiji Nambara, Mitsunori Seo, Hiroyuki Kasahara, Yusuke Jikumaru, Atsushi Hanada, and Yuri Kanno. We thank Dr. Shinjiro Yamaguchi for critical leading of this manuscript. MS is supported in part by the Japan Society for the Promotion of Science Grant-in-Aid for Young Scientists (B) (21770061).

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Authors and Affiliations

  1. Dormancy and Adaptation Research Unit, RIKEN Plant Science Center, Tsurumi-ku, Yokohama, Japan

    Mitsunori Seo

  2. Growth Regulation Research Group, RIKEN Plant Science Center, Yokohama, Kanagawa, Japan

    Yusuke Jikumaru & Yuji Kamiya

Authors
  1. Mitsunori Seo
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  2. Yusuke Jikumaru
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  3. Yuji Kamiya
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Corresponding author

Correspondence to Mitsunori Seo .

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Editors and Affiliations

  1. Dept. Biological Sciences, Simon Fraser Unviersity, University Drive 8888, Burnaby, V5A 1S6, British Columbia, Canada

    Allison R. Kermode

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Seo, M., Jikumaru, Y., Kamiya, Y. (2011). Profiling of Hormones and Related Metabolites in Seed Dormancy and Germination Studies. In: Kermode, A. (eds) Seed Dormancy. Methods in Molecular Biology, vol 773. Humana Press. https://doi.org/10.1007/978-1-61779-231-1_7

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  • DOI: https://doi.org/10.1007/978-1-61779-231-1_7

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-230-4

  • Online ISBN: 978-1-61779-231-1

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