Abstract
Here, we describe a series of methods suitable for the reproducible and abundant isolation of total RNA, genomic DNA, and total protein from dry or imbibed Arabidopsis seeds. The resulting material is suitable for most standard molecular biology procedures.
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Acknowledgments
Work in Luis Lopez-Molina’s laboratory is supported by the Swiss National Science Foundation and by the State of Geneva. We thank Olivier Schaad, Mylène Docquier, Patrick Descombes and members of the Genomics Platform of the National Research Center Frontiers in Genetics for conducting microarray experiment (Fig. 2).
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Piskurewicz, U., Lopez-Molina, L. (2011). Isolation of Genetic Material from Arabidopsis Seeds. In: Kermode, A. (eds) Seed Dormancy. Methods in Molecular Biology, vol 773. Humana Press. https://doi.org/10.1007/978-1-61779-231-1_10
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DOI: https://doi.org/10.1007/978-1-61779-231-1_10
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