Skip to main content

Dissection of Rodent Brain Regions

  • Protocol
  • First Online:
Neuroproteomics

Part of the book series: Neuromethods ((NM,volume 57))

Abstract

Dissection of brain tissue is an important step in sample preparation for (subcellular) proteomics studies. In this chapter, brain removal and separate dissection of multiple brain regions from a single brain are described in step-by-step protocol. This concerns dissection from fresh or frozen tissue of cerebellum, hippocampus, prefrontal cortex, and striatum.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Zhuo M, Hawkins RD (1995): Long-term depression: a learning-related type of synaptic plasticity in the mammalian central nervous system. Rev Neurosci 6:259–277.

    PubMed  CAS  Google Scholar 

  2. Teyler TJ, Discenna P (1984): Long-term potentiation as a candidate mnemonic device. Brain Res 319:15–28.

    PubMed  CAS  Google Scholar 

  3. Jorntell H, Hansel C (2006): Synaptic memories upside down: bidirectional plasticity at cerebellar parallel fiber-Purkinje cell synapses. Neuron 52:227–238.

    Article  PubMed  Google Scholar 

  4. Olsen JV, Nielsen PA, Andersen JR, Mann M, Wisniewski JR (2007): Quantitative proteomic profiling of membrane proteins from the mouse brain cortex, hippocampus, and cerebellum using the HysTag reagent: mapping of neurotransmitter receptors and ion channels. Brain Res 1134:95–106.

    Article  PubMed  CAS  Google Scholar 

  5. Trinidad JC, Thalhammer A, Specht CG, Lynn AJ, Baker PR, Schoepfer R, et al (2008): Quantitative analysis of synaptic phosphorylation and protein expression. Mol Cell Proteomics 7:684–696.

    PubMed  CAS  Google Scholar 

  6. Paxinos G, Franklin KBJ (2003): The mouse brain in stereotaxic coordinates: Academic press, San Diego.

    Google Scholar 

  7. Williams RW (1999): The Mouse Brain Library. http://www.mbl.org/atlas165/atlas165_start.html.

  8. Futterer CD, Maurer MH, Schmitt A, Feldmann RE, Jr., Kuschinsky W, Waschke KF (2004): Alterations in rat brain proteins after desflurane anesthesia. Anesthesiology 100:302–308.

    Article  PubMed  Google Scholar 

  9. Snyder GL, Galdi S, Hendrick JP, Hemmings HC, Jr. (2007): General anesthetics selectively modulate glutamatergic and dopaminergic signaling via site-specific phosphorylation in vivo. Neuropharmacology 53:619–630.

    Article  PubMed  CAS  Google Scholar 

  10. Westphalen RI, Hemmings HC, Jr. (2006): Volatile anesthetic effects on glutamate versus GABA release from isolated rat cortical nerve terminals: 4-aminopyridine-evoked release. J Pharmacol Exp Ther 316:216–223.

    Article  PubMed  CAS  Google Scholar 

  11. Westphalen RI, Hemmings HC, Jr. (2006): Volatile anesthetic effects on glutamate versus GABA release from isolated rat cortical nerve terminals: basal release. J Pharmacol Exp Ther 316:208–215.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Sabine Spijker .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Spijker, S. (2011). Dissection of Rodent Brain Regions. In: Li, K. (eds) Neuroproteomics. Neuromethods, vol 57. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-111-6_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-111-6_2

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-110-9

  • Online ISBN: 978-1-61779-111-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics