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Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods

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Mammalian Cell Viability

Part of the book series: Methods in Molecular Biology ((MIMB,volume 740))

Abstract

One of the traditional methods of cell viability analysis is the use of trypan blue dye exclusion staining. This technique has been the standard methodology used in academic research laboratories and industrial biotechnology plants. Cells were routinely counted manually with a hemocytometer. In recent years, modern automated instrumentation has been introduced to supplement this traditional technique with the efficiency and reproducibility of computer control, advanced imaging, and automated sample handling.

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References

  1. Cell and Tissue Culture: Laboratory Procedures. (1995) (Doyle, A., Griffiths, J.B., and Newell, D.G., Ed.). John Wiley & Sons, Inc., Chichester, England.

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  5. Innovatis Inc. Cedex. Web site: http://www.innovatis.com/pdf/download/brochures/Cedex.pdf.

  6. Louis, K.S., Siegel, A.C., and Levy, G.A. (2007) Comparison of Manual versus Automated Trypan Blue Dye Exclusion Method for Cell Counting. Web site: http://www.ibdl.ca/Application%20Notes/Application%20Note%20-%20Vi-CELL1.pdf.

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Author information

Authors and Affiliations

  1. MaRS Centre, Toronto Medical, University Health Network, Toronto, ON, Canada

    Andre C. Siegel

Authors
  1. Kristine S. Louis
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  2. Andre C. Siegel
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Corresponding author

Correspondence to Andre C. Siegel .

Editor information

Editors and Affiliations

  1. AO Research Institute, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland

    Martin J. Stoddart

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© 2011 Springer Science+Business Media, LLC

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Louis, K.S., Siegel, A.C. (2011). Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods. In: Stoddart, M. (eds) Mammalian Cell Viability. Methods in Molecular Biology, vol 740. Humana Press. https://doi.org/10.1007/978-1-61779-108-6_2

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  • DOI: https://doi.org/10.1007/978-1-61779-108-6_2

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-107-9

  • Online ISBN: 978-1-61779-108-6

  • eBook Packages: Springer Protocols

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