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Linearizer Gene Circuits with Negative Feedback Regulation

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Yeast Genetic Networks

Part of the book series: Methods in Molecular Biology ((MIMB,volume 734))

Abstract

Gene functional studies consist of phenotyping cells with altered gene expression. Improving the precision of current gene expression control techniques would enable more detailed studies of gene function. Here, we provide protocols for building synthetic gene constructs for tuning the expression of a gene in all the cells of a population precisely and uniformly, achieving expression levels proportional to the extracellular inducer concentration.

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Notes

  1. 1.

    http://www.async.ece.utah.edu/iBioSim/

  2. 2.

    http://www.r-project.org/

  3. 3.

    http://www.async.ece.utah.edu/iBioSim/

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Acknowledgments

We thank J. J. Collins for some of the constructs, yeast strains, and discussions. We also thank K. F. Murphy, K. Josić, R. Agarwal, T. Żal, A. Żal, G. Chodaczek, M. Stamatakis, W. Blake, T. F. Cooper, and B. Dutta for valuable comments and discussions. This work was supported by M. D. Anderson Cancer Center start-up funds.

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Authors and Affiliations

  1. UT M. D. Anderson Cancer Center, Houston, TX, USA

    Gábor Balázsi

Authors
  1. Dmitry Nevozhay
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  2. Rhys M. Adams
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  3. Gábor Balázsi
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Corresponding author

Correspondence to Gábor Balázsi .

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Editors and Affiliations

  1. Biochemisches Institut, Universität Zürich, Winterthurerstr. 190, Zürich, 8057, Switzerland

    Attila Becskei

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Nevozhay, D., Adams, R.M., Balázsi, G. (2011). Linearizer Gene Circuits with Negative Feedback Regulation. In: Becskei, A. (eds) Yeast Genetic Networks. Methods in Molecular Biology, vol 734. Humana Press. https://doi.org/10.1007/978-1-61779-086-7_5

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  • DOI: https://doi.org/10.1007/978-1-61779-086-7_5

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-085-0

  • Online ISBN: 978-1-61779-086-7

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