Skip to main content
SpringerLink
Log in

Wide Crossing in Lentil through Embryo Rescue

  • Protocol
  • First Online:
Plant Embryo Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 710))

Abstract

Lentil seeds have provided an appreciated source of protein, carbohydrates and fibre to the diet of humans since the dawn of agriculture. Low amounts of variation have been detected in the cultivated lentil germplasm collections. Interspecific crosses allow for the introgression of important alleles of agricultural interest from wild species, such as the resistance or tolerance to abiotic and biotic stresses. Interspecific crosses within the genus Lens generally abort and embryo rescue techniques are necessary to recover hybrids. The in vitro culture procedure to rescue interspecific hybrids of Lens consists of at least four different stages: (1) in ovulo embryo culture (2), embryo culture, (3) plantlet development and finally, (4) the gradual habituation to ex vitro conditions of the recovered interspecific hybrid plantlets. In this chapter, the approach to rescue interspecific hybrids in the genus Lens is outlined.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Ladizinsky G (1992) Crossability relations. In: Kalloo G, Chowdhury JB (eds) Distant hybridization of crop plants, monographs on theoretical and applied genetics 16. Springer, Dordrecht, pp 15–31

    Chapter  Google Scholar 

  2. Stebbins GL (1958) The inviability, weakness, and sterility of interspecific hybrids. Adv Genet 9:14–215

    Google Scholar 

  3. Yeung EC, Thorpe TA, Jensen CJ (1981) In vitro fertilization and embryo culture. In: Thorpe TA (ed) Plant tissue culture, methods and applications in agriculture. Academic, New York, pp 253–271

    Google Scholar 

  4. Abbo S, Ladizinsky G (1991) Anatomical aspects of hybrid embryo abortion in the genus Lens L. Bot Gaz 152:316–320

    Article  Google Scholar 

  5. Ladizinsky G (1993) Wild lentils. Crit Rev Plant Sci 12:169–184

    Google Scholar 

  6. Ladizinsky G, Abbo S (1993) Cryptic speciation in Lens culinaris. Genet Resourc Crop Evol 40:1–5

    Article  Google Scholar 

  7. Abbo S, Ladizinsky G (1994) Genetical aspects of hybrid embryo abortion in the genus Lens L. Heredity 72:193–200

    Article  Google Scholar 

  8. van Oss H, Aron Y, Ladizinsky G (1997) Chloroplast DNA variation and evolution in the genus Lens Mill. Theor Appl Genet 94:452–457

    Article  Google Scholar 

  9. Sandhu JS, Singh S (2007) History and origin. In: Yadav SS, McNeil D, Stevenson PC (eds) Lentil: an ancient crop for modern times. Springer, Dordrecht, pp 1–10

    Google Scholar 

  10. Cubero JI, Pérez de la Vega M, Fratini R (2009) Origin, phylogeny, domestication and spread. In: Erskine W, Muehlbauer F, Sharma B (eds) The lentil: botany, production and uses. CABI, Oxfordshire, pp 13–33

    Chapter  Google Scholar 

  11. Wilson VE (1972) Morphology and technique for crossing Lens esculenta Moench. Crop Sci 12:231–232

    Article  Google Scholar 

  12. Wilson VE, Law AG (1972) Natural crossing in Lens esculenta Moench. J Am Soc Hortic Sci 97:142–143

    Google Scholar 

  13. Horneburg B (2006) Outcrossing in lentil (Lens culinaris) depends on cultivar, location and year, and varies within cultivars. Plant Breed 125:638–640

    Article  Google Scholar 

  14. Alvarez MT, García P, Pérez de la Vega M (1997) RAPD polymorphism in Spanish lentil landraces and cultivars. J Genet Breed 51:91–96

    CAS  Google Scholar 

  15. Ford R, Pang ECK, Taylor PWJ (1997) Diversity analysis and species identification in Lens using PCR generated markers. Euphytica 96:247–255

    Article  CAS  Google Scholar 

  16. Ferguson ME, Newbury HJ, Maxted N, Ford-Lloyd BV, Robertson LD (1998) Population genetic structure in Lens taxa revealed by isozyme and RAPD analysis. Genet Resour Crop Evol 45:549–559

    Article  Google Scholar 

  17. Ferguson M (2000) Lens spp: conserved resources, priorities for collection and future prospects. In: Knight R (ed) Linking research and marketing opportunities for pulses in the 21st century, vol. 34, Current plant science and biotechnology in agriculture. Kluwer, Dordrecht, pp 613–620

    Chapter  Google Scholar 

  18. Sonante G, Pignone D (2001) Assessment of genetic variation in a collection of lentil using molecular tools. Euphytica 120:301–307

    Article  Google Scholar 

  19. Durán Y, Pérez de la Vega M (2004) Assessment of genetic variation and species relationships in a collection of Lens using RAPD and ISSR. Span J Agric Res 2:538–544

    Google Scholar 

  20. Erskine W, Tufail M, Russell A, Tyagi MC, Rahman MM, Saxena MC (1994) Current and future strategies in breeding lentil for resistance to biotic and abiotic stresses. Euphytica 73:127–135

    Article  Google Scholar 

  21. Ocampo B, Conicella C, Moss JP (2000) Wide crossing: opportunities and progress. In: Knight R (ed) Linking research and marketing opportunities for pulses in the 21st century, Vol. 34, Current plant science and biotechnology in agriculture. Kluwer, Dordrecht, pp 411–419

    Chapter  Google Scholar 

  22. Davis PA, Lülsdorf MM, Ahmad M (2007) Wild relatives and biotechnological appro-aches. In: Yadav SS, McNeil D, Stevenson PC (eds) Lentil: an ancient crop for modern times. Springer, Dordrecht, pp 225–240

    Google Scholar 

  23. Malhorta RS, Balyan HS, Gupta PK (1978) Crossing technique in lentils. Lens Newslet 5:7–8

    Google Scholar 

  24. Solh MB, Paredes OM, Tiwari AS (1980) Crossing technique in lentil under field conditions. Lens Newslet 7:9–14

    Google Scholar 

  25. Mera M, Erskine W (1982) Crossing techniques for lentil under field conditions. Lens Newslet 9:11–15

    Google Scholar 

  26. Kumar A, Singh DP (1998) Hybridization technique in lentil under field conditions. Lens Newslet 25:1–3

    Google Scholar 

  27. Fratini R, Ruiz ML, Pérez de la Vega M (2004) Intra-specific and inter-sub-specific crossing in lentil (Lens culinaris Medik.). Can J Plant Sci 84:981–986

    Article  Google Scholar 

  28. Ladizinky G (1979) The origin of lentil and its wild gene pool. Euphytica 28:179–187

    Article  Google Scholar 

  29. Ladizinsky G, Braun D, Goshen D, Muehlbauer FJ (1984) The biological species of the genus Lens L. (Lens nigricans). Bot Gaz 145:253–261

    Article  Google Scholar 

  30. Cohen D, Ladizinsky G, Ziv M, Muehlbauer FJ (1984) Rescue of interspecific Lens hybrids by means of embryo culture. Plant Cell Tiss Organ Cult 3:343–347

    Article  CAS  Google Scholar 

  31. Ladizinsky G, Cohen D, Muehlbauer FJ (1985) Hybridization in the genus Lens by means of embryo culture. Theor Appl Genet 70:97–101

    Article  Google Scholar 

  32. Fratini R, Ruiz ML (2006) Interspecific hybridization in the genus Lens applying in vitro embryo rescue. Euphytica 150:271–280

    Article  CAS  Google Scholar 

  33. Ahmad M, Fautrier AG, McNeil DL, Burritt DJ, Hill GD (1995) Attempts to overcome postfertilization barrier in interspecific crosses of the genus Lens. Plant Breed 114:558–560

    Article  Google Scholar 

  34. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  35. Biondi S, Thorpe TA (1981) Requirements for a tissue culture facility. In: Thorpe TA (ed) Plant tissue culture – methods and application in agriculture. Academic, NY, pp 1–20

    Google Scholar 

  36. Brown DCW, Thorpe TA (1984) Organization of a plant tissue culture laboratory. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, Vol. 1, Laboratory procedures and their applications. Academic, NY, pp 1–12

    Google Scholar 

  37. Pierik RLM (1998) In vitro culture of higher plants, 4th edn. Springer, Dordrecht

    Google Scholar 

  38. Muehlbauer FJ, Slinkard AE, Wilson VE (1980) Lentil. In: Fehr WR, Hadley HH (eds) Hybridization of crop plants. American Society of Agronomy and Crop Science Society of America, Madison, WI, pp 417–426

    Google Scholar 

  39. Fratini R, Ruiz ML (2008) Micropropagation of intra and interspecific Lens hybrids. Euphytica 159:199–206

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Biología Molecular, Area de Genética, Universidad de León, León, Spain

    Richard Fratini

  2. Area de Genética, Departamento de Biología Molecular, Universidad de León, León, Spain

    Maria L. Ruiz

Authors
  1. Richard Fratini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Maria L. Ruiz
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Richard Fratini .

Editor information

Editors and Affiliations

  1. Dept. Biological Sciences, University of Calgary, University Dr. NW., 2500, Calgary, T2N 1N4, Alberta, Canada

    Trevor A. Thorpe

  2. , Department of Biological Sciences, University of Calgary, University Dr. NW 2500, Calgary, T2N 1N4, Alberta, Canada

    Edward C. Yeung

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Humana Press

About this protocol

Cite this protocol

Fratini, R., Ruiz, M.L. (2011). Wide Crossing in Lentil through Embryo Rescue. In: Thorpe, T., Yeung, E. (eds) Plant Embryo Culture. Methods in Molecular Biology, vol 710. Humana Press. https://doi.org/10.1007/978-1-61737-988-8_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-61737-988-8_11

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61737-987-1

  • Online ISBN: 978-1-61737-988-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation