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Isolation and Culture of Human Multipotent Stromal Cells from the Pancreas

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Mesenchymal Stem Cell Assays and Applications

Part of the book series: Methods in Molecular Biology ((MIMB,volume 698))

Abstract

Mesenchymal stem cells, also termed multipotent mesenchymal stromal cells (MSCs), can be isolated from most adult tissues. Although the exact origin of MSCs expanded from the human pancreas has not been resolved, we have developed protocols to isolate and expand MSCs from human pancreatic tissue that remains after islet procurement. Similar to techniques used to isolate MSCs from bone marrow, pancreatic MSCs are isolated based on their cell adherence, expression of several cell surface antigens, and multilineage differentiation. The protocols for isolating, characterizing, and differentiating MSCs from the pancreas are presented in this chapter.

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Author information

Authors and Affiliations

  1. Alberta Diabetes Institute, University of Alberta, Edmonton, AB, Canada

    Gregory S. Korbutt

Authors
  1. Karen L. Seeberger
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  2. Alana Eshpeter
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  3. Gregory S. Korbutt
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Corresponding author

Correspondence to Gregory S. Korbutt .

Editor information

Editors and Affiliations

  1. , Primary and Stem Cell Systems, Life Technologies, Frederick, 21704, USA

    Mohan Vemuri

  2. Cellular Dynamics International, Inc., Science Drive 525, Madison, 53711, Wisconsin, USA

    Lucas G. Chase

  3. Life Technologies, Executive Way 7335, Frederick, 21704, Maryland, USA

    Mahendra S. Rao

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Seeberger, K.L., Eshpeter, A., Korbutt, G.S. (2011). Isolation and Culture of Human Multipotent Stromal Cells from the Pancreas. In: Vemuri, M., Chase, L., Rao, M. (eds) Mesenchymal Stem Cell Assays and Applications. Methods in Molecular Biology, vol 698. Humana Press. https://doi.org/10.1007/978-1-60761-999-4_10

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  • DOI: https://doi.org/10.1007/978-1-60761-999-4_10

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-998-7

  • Online ISBN: 978-1-60761-999-4

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