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Assessment of Nanomaterials Cytotoxicity and Internalization

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3D Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 695))

Abstract

The impact that nanotechnology may have on life and medical sciences is immense and includes novel therapies as much as novel diagnostic and imaging tools, often offering the possibility to combine the two. It is, therefore, of the essence to understand and control the interactions that nanomaterials can have with cells, first at an individual level, focusing on, e.g., binding and internalization events, and then at a tissue level, where diffusion and long-range transport add further complications. Here, we present experimental methods based on selective labeling techniques and the use of effectors for a qualitative and quantitative evaluation of endocytic phenomena involving nanoparticles. The understanding of the cell–material interactions arising from these tests can then form the basis for a model-based evaluation of nanoparticles behavior in 3D tissues.

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Acknowledgments

This work was supported by a Science and Technology Development Fund (STDF) grant to Noha M. Zaki.

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Authors and Affiliations

  1. Department of Pharmaceutics, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt

    Noha M. Zaki

  2. School of Pharmacy and Pharmaceutical Sciences, The University of Manchester, Manchester, UK

    Nicola Tirelli

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  1. Noha M. Zaki
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  2. Nicola Tirelli
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Editors and Affiliations

  1. Kroto Research Institute, Department of Engineering Materials, University of Sheffield, Broad Lane, Sheffield, S3 7HW, United Kingdom

    John W. Haycock

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Zaki, N.M., Tirelli, N. (2011). Assessment of Nanomaterials Cytotoxicity and Internalization. In: Haycock, J. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 695. Humana Press. https://doi.org/10.1007/978-1-60761-984-0_16

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  • DOI: https://doi.org/10.1007/978-1-60761-984-0_16

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  • Print ISBN: 978-1-60761-983-3

  • Online ISBN: 978-1-60761-984-0

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