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Construction of Gene Interruptions and Gene Deletions in the Cyanobacterium Synechocystis sp. Strain PCC 6803

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Photosynthesis Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 684))

Abstract

A series of protocols are presented for the storage, growth, transformation, and characterization of wild type and mutant strains of Synechocystis sp. strain PCC 6803. These protocols include the isolation of genomic DNA and the strategies required for the construction of specific gene interruptions or deletions in this organism. This cyanobacterium has been used widely as a model for photosynthesis research, and the sequence of its genome is available at CyanoBase (http://genome.kazusa.or.jp/cyanobase/). The details provided in this chapter do not assume any previous experience in working with cyanobacteria and are intended to enable new investigators to take advantage of a wide range of gene modification and mutation mapping techniques that have been adapted for use in this system.

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Acknowledgment

The author wishes to thank Professor Wim Vermaas as many of the procedures described in this chapter are based on methods that the author originally acquired in the Vermaas laboratory at Arizona State University. The methods for the isolation of genomic DNA are modified from an original procedure designed by Dr. J. G. K. Williams.

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Authors and Affiliations

  1. Biochemistry Department, University of Otago, Dunedin, New Zealand

    Julian J. Eaton-Rye

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  1. Julian J. Eaton-Rye
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Correspondence to Julian J. Eaton-Rye .

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  1. , Département de Chimie Biologie, Université du Québec à Trois-Rivières, Boul. des Forges 3351, Trois-Rivieres, G9A5H7, Québec, Canada

    Robert Carpentier

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Eaton-Rye, J.J. (2011). Construction of Gene Interruptions and Gene Deletions in the Cyanobacterium Synechocystis sp. Strain PCC 6803. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods in Molecular Biology, vol 684. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-925-3_22

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  • DOI: https://doi.org/10.1007/978-1-60761-925-3_22

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-924-6

  • Online ISBN: 978-1-60761-925-3

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