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Intracellular Delivery of Nanoparticles with CPPs

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Cell-Penetrating Peptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 683))

Abstract

Cell-penetrating peptides (CPPs), in particular TATp, have been widely used for intracellular delivery of various cargoes, both in vitro and in vivo. Modifications of nanoparticles with CPPs require either covalent or noncovalent approach. Here we describe various methods to attach CPP, such as TATp to surface of nanocarriers (such as liposomes and micelles), loading with drug or DNA and characterization of same for in vitro and in vivo applications. Due to nonselectivity of CPPs and wide distribution in nontarget areas, method for preparation of “smart” nanocarrier with hidden TATp function is also described.

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Authors and Affiliations

  1. Research Associate Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, USA

    Rupa Sawant

  2. Center for Pharmaceutical, Biotechnology and Nanomedicine, Northeastern University, Mugar Building, Boston, MA, USA

    Vladimir Torchilin

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  2. Vladimir Torchilin
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  1. , Department of Neurochemistry, Stockholm University, Svante Arrheniusv 21A21A, Stockholm, 106 91, Sweden

    Ülo Langel

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© 2011 Springer Science+Business Media, LLC

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Sawant, R., Torchilin, V. (2011). Intracellular Delivery of Nanoparticles with CPPs. In: Langel, Ü. (eds) Cell-Penetrating Peptides. Methods in Molecular Biology, vol 683. Humana Press. https://doi.org/10.1007/978-1-60761-919-2_31

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  • DOI: https://doi.org/10.1007/978-1-60761-919-2_31

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-918-5

  • Online ISBN: 978-1-60761-919-2

  • eBook Packages: Springer Protocols

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