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Methods for In Vitro Generation of Human Type 1 Regulatory T Cells

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Suppression and Regulation of Immune Responses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 677))

Abstract

Type 1 regulatory T (Tr1) cells are adaptive regulatory T cells that are induced in the periphery upon chronic exposure to antigen (Ag) in a tolerogenic environment containing interleukin (IL)-10. Tr1 cells are Ag-specific; they produce high levels of IL-10 and TGF-β in the absence of IL-4 and suppress T-cell responses via a cytokine-dependent mechanism. During the last decade, several protocols have been developed to generate Tr1 cell lines in vitro. In this chapter, we outline protocols to generate non-Ag- and Ag-specific Tr1 cell lines and assays used to characterize Tr1 cell phenotype and functions.

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Acknowledgments

The authors thank Claudia Sartirana and Chiara Francesca Magnani for technical help. This work was supported by a grant from the Italian Telethon Foundation, Cariplo Foundation, the Italian Association for Cancer Research (Associazione Italiana per la Ricerca sul Cancro, AIRC), and the EU graut to MGR, Riset Consortion (www.risetFp6.org)

Author information

Authors and Affiliations

  1. Department of Regenerative Medicine, Stem Cells, and Gene Therapy, San Raffaele Telethon Institute for Gene Therapy (HSR-TIGET), Milan, Italy

    Silvia Gregori, Maria Grazia Roncarolo & Rosa Bacchetta

  2. Universita’ Vita-Salute San Raffaele, Milan, Italy

    Maria Grazia Roncarolo

Authors
  1. Silvia Gregori
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  2. Maria Grazia Roncarolo
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  3. Rosa Bacchetta
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Corresponding author

Correspondence to Rosa Bacchetta .

Editor information

Editors and Affiliations

  1. CHUHotel Dieu, INSERM UMR 643, Bd. Jean Monnet 30, Nantes, 44093, France

    Maria Cristina Cuturi

  2. CHU Hotel Dieu, INSERM UMR 643, Bd. Jean Monnet 30, Nantes, 44093, France

    Ignacio Anegon

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© 2010 Humana Press

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Gregori, S., Roncarolo, M.G., Bacchetta, R. (2010). Methods for In Vitro Generation of Human Type 1 Regulatory T Cells. In: Cuturi, M., Anegon, I. (eds) Suppression and Regulation of Immune Responses. Methods in Molecular Biology, vol 677. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-869-0_3

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  • DOI: https://doi.org/10.1007/978-1-60761-869-0_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-868-3

  • Online ISBN: 978-1-60761-869-0

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