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Construction and Screening of Marine Metagenomic Libraries

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Metagenomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 668))

Abstract

Marine microbial communities are highly diverse and have evolved during extended evolutionary processes of physiological adaptations under the influence of a variety of ecological conditions and selection pressures. They harbor an enormous diversity of microbes with still unknown and probably new physiological characteristics. Besides, the surfaces of marine multicellular organisms are typically covered by a consortium of epibiotic bacteria and act as barriers, where diverse interactions between microorganisms and hosts take place. Thus, microbial diversity in the water column of the oceans and the microbial consortia on marine tissues of multicellular organisms are rich sources for isolating novel bioactive compounds and genes. Here we describe the sampling, construction of large-insert metagenomic libraries from marine habitats and exemplarily one function based screen of metagenomic clones.

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Author information

Authors and Affiliations

  1. Institute for General Microbiology, Christian Albrechts University Kiel, Kiel, Germany

    Nancy Weiland, Carolin Löscher, Rebekka Metzger & Ruth Schmitz

Authors
  1. Nancy Weiland
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  2. Carolin Löscher
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  3. Rebekka Metzger
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  4. Ruth Schmitz
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Corresponding author

Correspondence to Ruth Schmitz .

Editor information

Editors and Affiliations

  1. Biocenter Klein Flottbek, Microbiology & Biotechnology, University of Hamburg, Ohnhorststr. 18, Hamburg, 22609, Germany

    Wolfgang R. Streit

  2. Institute of Microbiology and Genetics, Dept. of Genomic & Applied Microbiology, Georg August University, Grisebachstraße 8, Göttingen, 37077, Germany

    Rolf Daniel

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© 2010 Humana Press

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Weiland, N., Löscher, C., Metzger, R., Schmitz, R. (2010). Construction and Screening of Marine Metagenomic Libraries. In: Streit, W., Daniel, R. (eds) Metagenomics. Methods in Molecular Biology, vol 668. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-823-2_3

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  • DOI: https://doi.org/10.1007/978-1-60761-823-2_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-822-5

  • Online ISBN: 978-1-60761-823-2

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