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Beyond DNA: Zinc Finger Domains as RNA-Binding Modules

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Engineered Zinc Finger Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 649))

Abstract

Over the last 25 years, we have learned that many structural classes of zinc-binding domains (zinc fingers, ZFs) exist and it has become clear that the molecular functions of these domains are by no means limited to the sequence-specific recognition of double-stranded DNA. For example, ZFs can act as protein recognition or RNA-binding modules, and some domains can exhibit more than one function. In this chapter we describe the progress that has been made in understanding the role of ZF domains as RNA-recognition modules, and we speculate about both the prevalence of such functions and the prospects for creating designer ZFs that target RNA.

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  1. School of Molecular Bioscience, University of Sydney, Sydney, NSW, Australia

    Josep Font & Joel P. Mackay

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  1. Josep Font
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  2. Joel P. Mackay
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Editors and Affiliations

  1. School of Molecular &, Microbial Biosciences, University of Sydney, Cnr. Butlin Ave. & Maze Cres., Sydney, 2006, New South Wales, Australia

    Joel P. Mackay

  2. Dept. Pharmacology & Genome Center, University of California, Davis, E. Health Sciences Drive 451, Davis, 95616, California, USA

    David J. Segal

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Font, J., Mackay, J.P. (2010). Beyond DNA: Zinc Finger Domains as RNA-Binding Modules. In: Mackay, J., Segal, D. (eds) Engineered Zinc Finger Proteins. Methods in Molecular Biology, vol 649. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-753-2_29

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  • DOI: https://doi.org/10.1007/978-1-60761-753-2_29

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