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Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches

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Cellular Programming and Reprogramming

Part of the book series: Methods in Molecular Biology ((MIMB,volume 636))

Abstract

Although embryonic stem cells (ESCs) have been established from mice since 1981, attempts to derive its counterparts from various other mammals, including rats, have not succeeded. Recently, induced pluripotent stem cells (iPSCs) have been generated from both mouse and human somatic cells by genetic transduction. We had successfully established novel rat iPSCs (riPSCs), which can be homogenously maintained by LIF and a cocktail of ALK5 inhibitor, GSK3 inhibitor and MEK inhibitor. riPSCs share conventional mouse ESC characteristics and most importantly can contribute extensively to chimeras. We also generated novel human iPSCs (hiPSCs) with “mouse ESC-like” characteristics, which can be surprisingly maintained in culture in the presence of MEK inhibitor and ALK5 inhibitor.

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Authors and Affiliations

  1. Department of Chemistry, The Scripps Research Institute, La Jolla, CA, USA

    Wenlin Li & Sheng Ding

Authors
  1. Wenlin Li
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  2. Sheng Ding
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Corresponding author

Correspondence to Sheng Ding .

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Editors and Affiliations

  1. The Scripps Research Institute, N. Torrey Pines Road 10550, La Jolla, 92037, U.S.A.

    Sheng Ding

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Li, W., Ding, S. (2010). Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches. In: Ding, S. (eds) Cellular Programming and Reprogramming. Methods in Molecular Biology, vol 636. Humana Press. https://doi.org/10.1007/978-1-60761-691-7_18

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  • DOI: https://doi.org/10.1007/978-1-60761-691-7_18

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-690-0

  • Online ISBN: 978-1-60761-691-7

  • eBook Packages: Springer Protocols

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