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Activation Tagging with En/Spm-I /dSpm Transposons in Arabidopsis

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Plant Reverse Genetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 678))

Abstract

Activation tagging is a powerful strategy to find new gene functions, especially from genes that are redundant or show lethal knock-out phenotypes. It has been applied using T-DNA or transposons. En/Spm-I/dSpm engineered transposons are efficient Activation tags in Arabidopsis. An immobilized transposase source and an enhancer-bearing non-autonomous element are used in combination with positive and negative selectable markers to generate a population of single or low copy, stable insertions. This method describes the steps required to select the best parental lines, generate a population of stable insertions, and gene identification.

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Authors and Affiliations

  1. Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, México

    Nayelli Marsch-Martínez

  2. Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA, USA

    Andy Pereira

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  1. Nayelli Marsch-Martínez
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  2. Andy Pereira
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  1. , Virginia Bioinformatics Institute, Virginia Tech, Washington Street, Blacksburg, 24061, Maryland, USA

    Andy Pereira

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Marsch-Martínez, N., Pereira, A. (2011). Activation Tagging with En/Spm-I /dSpm Transposons in Arabidopsis . In: Pereira, A. (eds) Plant Reverse Genetics. Methods in Molecular Biology, vol 678. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-682-5_8

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  • DOI: https://doi.org/10.1007/978-1-60761-682-5_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-681-8

  • Online ISBN: 978-1-60761-682-5

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